Size characterization of magnetic cell sorting microbeads using flow field-flow fractionation and photon correlation spectroscopy

被引:10
|
作者
Williams, SKR [1 ]
Lee, H [1 ]
Turner, MM [1 ]
机构
[1] Colorado Sch Mines, Dept Chem & Geochem, Golden, CO 80401 USA
关键词
field-flow fractionation; photon correlation spectroscopy;
D O I
10.1016/S0304-8853(98)00551-4
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Flow field-flow fractionation (FFF) and photon correlation spectroscopy (PCS) were used to determine the size distribution of magnetic cell sorting (MACS) microbeads. The nominal 50 nm microbeads, when coupled to monoclonal Anti-Fluorescein isothiocyanate (FITC), have an average diameter of 125 nm. FFF results showed that two different batches of microbeads had similar average diameters. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:248 / 253
页数:6
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