Characterization and stabilization of solubilized membrane proteins

被引：0
|
作者：
Ebel, Ch. [1 ]
机构：
[1] Univ Grenoble 1, CNRS, CEA, Inst Biol Struct,Lab Biophys Mol, Grenoble, France
来源：
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 2008年 / 22卷 / 01期
关键词：
membrane proteins; analytical ultracentrifugation; small angle neutron scattering; detergent; stabilization;
D O I：
10.1080/13102818.2008.10817522
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We present briefly the principles of analytical ultracentrifugation (AUC), density measurements, and small angle neutron scattering (SANS). These techniques are used to characterize the solubilized membrane proteins assemblies. We show how protein-detergent and protein-protein interactions can be linked in a thermodynamical approach. In conclusion we review our recent results concerning the use of original surfactants for the stabilization of membrane proteins in solution.
引用
收藏
页码：620 / 624
页数：5
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