Visual detection of cysteine and homocysteine

被引：489
作者：
Rusin, O
St Luce, NN
Agbaria, RA
Escobedo, JO
Jiang, S
Warner, IM
Dawan, FB
Lian, K
Strongin, RM [1 ]
机构：
[1] Louisiana State Univ, Dept Chem, Baton Rouge, LA 70803 USA
[2] Louisiana State Univ, Ctr Adv Microstruct & Devices, Baton Rouge, LA 70803 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 02期
关键词：
D O I：
10.1021/ja036297t
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The determination of cysteine and homocysteine levels is of great current interest for the monitoring of desease states. A new colorimetric method for the simultaneous detection of L-cysteine and L-homocysteine has been developed. A fluorescein derivative reacts with the above amino acids, producing their respective thiazolidines resulting in color changes. Interference from other amino acids and proteins is minimal. Copyright © 2004 American Chemical Society.
引用
收藏
页码：438 / 439
页数：2
相关论文
共 19 条
[1]   Specific determination of cysteine and penicillamine through cyclization to 2-thioxothiazolidine-4-carboxylic acids [J].
Amarnath, V ;
Amarnath, K .
TALANTA, 2002, 56 (04) :745-751
[2]   Fluorescent sensors for Zn2+ based on a fluorescein platform:: Synthesis, properties and intracellular distribution [J].
Burdette, SC ;
Walkup, GK ;
Spingler, B ;
Tsien, RY ;
Lippard, SJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (32) :7831-7841
[3]   Assays for total homocysteine and other thiols by capillary electrophoresis-laser-induced fluorescence detection I.: Preanalytical condition studies [J].
Caussé, E ;
Issac, C ;
Malatray, P ;
Bayle, C ;
Valdiguié, P ;
Salvayre, R ;
Couderc, F .
JOURNAL OF CHROMATOGRAPHY A, 2000, 895 (1-2) :173-178
[4]  
COOPER AJL, 1982, J BIOL CHEM, V257, P816
[5]   Simple and rapid visual sensing of saccharides [J].
Davis, CJ ;
Lewis, PT ;
McCarroll, ME ;
Read, MW ;
Cueto, R ;
Strongin, RM .
ORGANIC LETTERS, 1999, 1 (02) :331-334
[6]   Determination of biological thiols by high-performance liquid chromatography following derivatization by ThioGlo maleimide reagents [J].
Ercal, N ;
Yang, P ;
Aykin, N .
JOURNAL OF CHROMATOGRAPHY B, 2001, 753 (02) :287-292
[7]  
FOURNEAU JP, 1971, CR ACAD SCI C CHIM, V272, P1982
[8]   Chromophore formation in resorcinarene solutions and the visual detection of mono- and oligosaccharides [J].
He, M ;
Johnson, RJ ;
Escobedo, JO ;
Beck, PA ;
Kim, KK ;
St Luce, NN ;
Davis, CJ ;
Lewis, PT ;
Fronczek, FR ;
Melancon, BJ ;
Mrse, AA ;
Treleaven, WD ;
Strongin, RM .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (18) :5000-5009
[9]   Qualitative and quantitative determination of biologically active low-molecular-mass thiols in human blood by reversed-phase high-performance liquid chromatography with photometry and fluorescence detection [J].
Ivanov, AR ;
Nazimov, IV ;
Baratova, LA .
JOURNAL OF CHROMATOGRAPHY A, 2000, 870 (1-2) :433-442
[10]   Visual sensing of saccharides promoted by resorcinol condensation products [J].
Lewis, PT ;
Davis, CJ ;
Cabell, LA ;
He, M ;
Read, MW ;
McCarroll, ME ;
Strongin, RM .
ORGANIC LETTERS, 2000, 2 (05) :589-592
12