Covalent Immobilization of Candida rugosa Lipase on Epichlorohydrin-Coated Magnetite Nanoparticles: Enantioselective Hydrolysis Studies of Some Racemic Esters and HPLC Analysis

被引：11

作者：

Cakmak, Resit ^{[1
]}

Topal, Giray ^{[2
]}

Cinar, Ercan ^{[3
]}

机构：

[1] Batman Univ, Vocat Sch Hlth Serv, Med Lab Tech Program, TR-72060 Batman, Turkey

[2] Dicle Univ, Fac Educ, Dept Chem, TR-21280 Diyarbakir, Turkey

[3] Batman Univ, Sch Hlth Sci, Dept Nursing, TR-72060 Batman, Turkey

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2020年 / 191卷 / 04期

关键词：

Fe3O4; Lipase; Immobilization; Biocatalysis; Racemic esters; Hydrolysis; HPLC; METAL-ORGANIC FRAMEWORKS; IRON-OXIDE NANOPARTICLES; HOLLOW-FIBER MEMBRANE; NAPROXEN METHYL-ESTER; ENZYME IMMOBILIZATION; BIODIESEL PRODUCTION; KINETIC RESOLUTION; INDUSTRIAL APPLICATIONS; CATALYZED HYDROLYSIS; PURIFICATION;

D O I：

10.1007/s12010-020-03274-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, a new biocatalyst was prepared by immobilizing Candida rugosa lipase epichlorohydrin-functionalized onto the surface of the nanoparticles. Magnetite nanoparticles were obtained by chemical co-precipitation method of Fe2+ and Fe3+, and then the prepared uncoated and coated nanoparticles were characterized by XRD, FT-IR and TGA. Lipase was covalently attached to activated nanoparticles. The catalytic properties of free and immobilized lipases were determined. It was found that the optimum temperature for free and immobilized lipases was 30 degrees C and 35 degrees C, respectively. The optimum pH values were found to be 7.0 and 8 for free and immobilized lipases, respectively. Immobilized lipase was found to retain significant activity even after the seventh use. In the final section of the study, optically pure compounds were obtained by carrying out the enantioselective hydrolysis studies of racemic esters by using immobilized lipase. Enantiomeric excesses of the products in the enantioselective hydrolysis of racemic ibuprofen and naproxen methyl ester and racemic butyl mandelate were determined to be 94.93, 77.30 and 68.15, respectively.

引用

页码：1411 / 1431

页数：21

共 101 条

[1] Characterization of an anion-exchange porous polypropylene hollow fiber membrane for immobilization of ABL lipase [J].

Abrol, Kavita ;

Qazi, G. N. ;

Ghosh, Anup K. .

JOURNAL OF BIOTECHNOLOGY, 2007, 128 (04) :838-848

[2] Reversible immobilization of tyrosinase onto polyethyleneimine-grafted and Cu(II) chelated poly(HEMA-co-GMA) reactive membranes [J].

Arica, MY ;

Bayramoglu, G .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2004, 27 (4-6) :255-265

[3]

Arica MY, 2000, J APPL POLYM SCI, V77, P2000, DOI 10.1002/1097-4628(20000829)77:9<2000::AID-APP16>3.0.CO

[4]

2-M

[5] PREPARATION OF MODIFIED AGAROSE GELS CONTAINING THIOL-GROUPS [J].

AXEN, R ;

DREVIN, H ;

CARLSSON, J .

ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY, 1975, 29 (04) :471-474

[6] Terminology of metal-organic frameworks and coordination polymers (IUPAC Recommendations 2013) [J].

Batten, Stuart R. ;

Champness, Neil R. ;

Chen, Xiao-Ming ;

Garcia-Martinez, Javier ;

Kitagawa, Susumu ;

Ohrstrom, Lars ;

O'Keeffe, Michael ;

Suh, Myunghyun Paik ;

Reedijk, Jan .

PURE AND APPLIED CHEMISTRY, 2013, 85 (08) :1715-1724

[7] ENZYMATIC RESOLUTION OF (S)-(+)-NAPROXEN IN A CONTINUOUS REACTOR [J].

BATTISTEL, E ;

BIANCHI, D ;

CESTI, P ;

PINA, C .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (06) :659-664

[8] Enzymatic resolution of substituted mandelic acids [J].

Campbell, RF ;

Fitzpatrick, K ;

Inghardt, T ;

Karlsson, O ;

Nilsson, K ;

Reilly, JE ;

Yet, L .

TETRAHEDRON LETTERS, 2003, 44 (29) :5477-5481

[9] Magnetic AuNP@Fe3O4 nanoparticles as reusable carriers for reversible enzyme immobilization [J].

Cao, Yao ;

Wen, Liyin ;

Svec, Frantisek ;

Tan, Tianwei ;

Lv, Yongqin .

CHEMICAL ENGINEERING JOURNAL, 2016, 286 :272-281

[10] Novel microbial lipases: catalytic activity in reactions in organic media [J].

Cardenas, F ;

de Castro, MS ;

Sanchez-Montero, JM ;

Sinisterra, JV ;

Valmaseda, M ;

Elson, SW ;

Alvarez, E .

ENZYME AND MICROBIAL TECHNOLOGY, 2001, 28 (2-3) :145-154

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