Obtaining and characterization of EF-hand mutants of recoverin

被引：19
|
作者：
Alekseev, AM
Shulga-Morskoy, SV
Zinchenko, DV
Shulga-Morskaya, SA
Suchkov, DV
Vaganova, SA
Senin, II
Zargarov, AA
Lipkin, VM
Akhtar, M
Philippov, PP [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Dept Enzymol, Moscow 119899, Russia
[2] Branch Shemyakin & Ovchinnikov Inst Bioorgan Chem, Pushchino 142292, Moscow Region, Russia
[3] Univ Southampton, Dept Biochem, Southampton SO9 3TU, Hants, England
来源：
FEBS LETTERS | 1998年 / 440卷 / 1-2期
基金：
英国惠康基金;
关键词：
photoreception; phosphorylation; rhodopsin; rhodopsin kinase; recoverin; calcium-binding protein; retinal rod cell;
D O I：
10.1016/S0014-5793(98)01426-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Several EF-hand recoverin mutants mere obtained and their abilities to bind to photoreceptor membranes and to inhibit rhodopsin kinase mere determined. The mutants with the 'spoiled' 2nd, 3rd or (2nd+3rd) EF-hand structures did not act upon the kinase activity in the mu M range of Ca2+ concentrations. Mutations of the 4th EF hand, which 'repaired' its Ca2+-binding activity, resulted in recoverin with three 'working' Ca2+-binding sites. The latter mutant inhibited rhodopsin kinase even more effectively than the wild-type recoverin, containing two working Ca2+-binding structures. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：116 / 118
页数：3
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