Obtaining and characterization of EF-hand mutants of recoverin

被引：19

作者：

Alekseev, AM

Shulga-Morskoy, SV

Zinchenko, DV

Shulga-Morskaya, SA

Suchkov, DV

Vaganova, SA

Senin, II

Zargarov, AA

Lipkin, VM

Akhtar, M

Philippov, PP ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Dept Enzymol, Moscow 119899, Russia

[2] Branch Shemyakin & Ovchinnikov Inst Bioorgan Chem, Pushchino 142292, Moscow Region, Russia

[3] Univ Southampton, Dept Biochem, Southampton SO9 3TU, Hants, England

来源：

FEBS LETTERS | 1998年 / 440卷 / 1-2期

基金：

英国惠康基金;

关键词：

photoreception; phosphorylation; rhodopsin; rhodopsin kinase; recoverin; calcium-binding protein; retinal rod cell;

D O I：

10.1016/S0014-5793(98)01426-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several EF-hand recoverin mutants mere obtained and their abilities to bind to photoreceptor membranes and to inhibit rhodopsin kinase mere determined. The mutants with the 'spoiled' 2nd, 3rd or (2nd+3rd) EF-hand structures did not act upon the kinase activity in the mu M range of Ca2+ concentrations. Mutations of the 4th EF hand, which 'repaired' its Ca2+-binding activity, resulted in recoverin with three 'working' Ca2+-binding sites. The latter mutant inhibited rhodopsin kinase even more effectively than the wild-type recoverin, containing two working Ca2+-binding structures. (C) 1998 Federation of European Biochemical Societies.

引用

页码：116 / 118

页数：3

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