Affinity-based tagging of protein families with reversible inhibitors: A concept for functional proteomics

被引：43
作者：
Hagenstein, MC
Mussgnug, JH
Lotte, K
Plessow, R
Brockhinke, A
Kruse, O
Sewald, N
机构：
[1] Univ Bielefeld, Dept Biol, D-33615 Bielefeld, Germany
[2] Univ Bielefeld, Dept Chem Organ & Bioorgan Chem, D-33615 Bielefeld, Germany
[3] Univ Bielefeld, Dept Chem, D-33615 Bielefeld, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 45期
关键词：
fluorescent probes; inhibitors; kinases; photoaffinity labeling; proteomics;
D O I：
10.1002/anie.200352084
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Kinases can be tagged with an engineered chemical probe that comprises a reversibly binding protein ligand, a photoreactive group (4-benzoylphenylalanine), and a fluorescent reporter group (carboxyfluorescein). This principle, which does not require irreversible inhibitors, allows affinity-based detection of protein families in functional proteomics.
引用
收藏
页码：5635 / 5638
页数：4
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