Serum concentrations of lectin-pathway components in healthy neonates, children and adults: mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2)

被引：88

作者：

Sallenbach, Seraina ^{[1
]}

Thiel, Steffen ^{[2
]}

Aebi, Christoph ^{[1
,3
]}

Otth, Margrith ^{[1
]}

Bigler, Susanna ^{[3
]}

Jensenius, Jens C. ^{[2
]}

Schlapbach, Luregn J. ^{[1
]}

Ammann, Roland A. ^{[1
]}

机构：

[1] Univ Bern, Dept Pediat, CH-3010 Bern, Switzerland

[2] Univ Aarhus, Dept Med Microbiol & Immunol, Aarhus, Denmark

[3] Univ Bern, Inst Infect Dis, CH-3010 Bern, Switzerland

来源：

PEDIATRIC ALLERGY AND IMMUNOLOGY | 2011年 / 22卷 / 04期

关键词：

complement; healthy children; innate immunity; lectin pathway; normal range; PATTERN-RECOGNITION MOLECULES; CORD BLOOD-LEVELS; COMPLEMENT; DEFICIENCY; SUSCEPTIBILITY; INFECTION; INFANTS; PROTEIN; FEVER; RISK;

D O I：

10.1111/j.1399-3038.2010.01104.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

P>This study aimed to measure serum concentrations of five lectin-pathway components, mannan-binding lectin (MBL), M-ficolin, L-ficolin, H-ficolin, and MBL-associated serine protease-2 (MASP-2), in healthy neonates and children, to determine if they change with age and to compare them with serum concentrations in healthy adults. Concentrations were measured in 141 preterm and 30 term neonates, in 120 children including infants and adolescents, and in 350 adults (97 for L-ficolin) by inhouse time-resolved immunofluorometric assays or commercially available enzyme-linked immunosorbent assays. The adjacent categories method applying Wilcoxon-Mann-Whitney tests was used to determine age categories where concentrations differed significantly. Displaying serum concentration vs. age, an inverted-U shape (higher concentrations in children than in neonates and adults) was found for MBL and the ficolins, and an S-shape for MASP-2. Serum concentrations of all five lectin-pathway components were significantly lower in preterm neonates < 32-wk gestational age compared to older neonates, infants, and children. Only M-ficolin in children > 1 yr and H-ficolin in term neonates and in children were found to be comparable with adult values. MBL, M-, L-, and H-ficolin, and MASP-2 serum concentrations show important changes with age. The respective normal ranges for adults should not be used in the pediatric population. The age-specific pediatric ranges established here may be used instead.

引用

页码：424 / 430

页数：7

共 30 条

[21] Differential Role of the Lectin Pathway of Complement Activation in Susceptibility to Neonatal Sepsis [J].

Schlapbach, Luregn J. ;

Mattmann, Maika ;

Thiel, Steffen ;

Boillat, Colette ;

Otth, Margrith ;

Nelle, Mathias ;

Wagner, Bendicht ;

Jensenius, Jens C. ;

Aebi, Christoph .

CLINICAL INFECTIOUS DISEASES, 2010, 51 (02) :153-162

[22] M-ficolin in the neonatal period: Associations with need for mechanical ventilation and mortality in premature infants with necrotising enterocolitis [J].

Schlapbach, Luregn J. ;

Kessler, Ulf ;

Thiel, Steffen ;

Hansen, Annette G. ;

Nelle, Mathias ;

Ammann, Roland A. ;

Aebi, Christoph ;

Jensenius, Jens C. .

MOLECULAR IMMUNOLOGY, 2009, 46 (13) :2597-2603

[23] Mannose-binding lectin cord blood levels and respiratory symptoms during infancy: a prospective birth cohort study [J].

Schlapbach, Luregn Jan ;

Latzin, Philipp ;

Regamey, Nicolas ;

Kuehni, Claudia E. ;

Zwahlen, Marcel ;

Casaulta, Carmen ;

Aebi, Christoph ;

Frey, Urs .

PEDIATRIC ALLERGY AND IMMUNOLOGY, 2009, 20 (03) :219-226

[24] Inherited deficiency of mannan-binding lectin-associated serine protease 2 [J].

Stengaard-Pedersen, K ;

Thiel, S ;

Gadjeva, M ;

Moller-Kristensen, M ;

Sorensen, R ;

Jensen, LT ;

Sjoholm, AG ;

Fugger, L ;

Jensenius, JC .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 349 (06) :554-560

[25] Association of mutations in mannose binding protein gene with childhood infection in consecutive hospital series [J].

Summerfield, JA ;

Sumiya, M ;

Levin, M ;

Turner, MW .

BRITISH MEDICAL JOURNAL, 1997, 314 (7089) :1229-1232

[26] Two factors of the lectin pathway of complement, L-ficolin and mannan-binding lectin, and their associations with prematurity, low birthweight and infections in a large cohort of Polish neonates [J].

Swierzko, Anna St. ;

Atkinson, Anne P. M. ;

Cedzynski, Maciej ;

MacDonald, Shirley L. ;

Szala, Agnieszka ;

Domzalska-Popadiuk, Iwona ;

Borkowska-Klos, Monika ;

Jopek, Aleksandra ;

Szczapa, Jerzy ;

Matsushita, Misao ;

Szemraj, Janusz ;

Turner, Marc L. ;

Kilpatrick, David C. .

MOLECULAR IMMUNOLOGY, 2009, 46 (04) :551-558

[27] Complement activating soluble pattern recognition molecules with collagen-like regions, mannan-binding lectin, ficolins and associated proteins [J].

Thiel, Steffen .

MOLECULAR IMMUNOLOGY, 2007, 44 (16) :3875-3888

[28] Characteristics and Biological Variations of M-Ficolin, a Pattern Recognition Molecule, in Plasma [J].

Wittenborn, Thomas ;

Thiel, Steffen ;

Jensen, Lisbeth ;

Nielsen, Hans J. ;

Jensenius, Jens C. .

JOURNAL OF INNATE IMMUNITY, 2010, 2 (02) :167-180

[29] The development of the complement system after 28 weeks' gestation [J].

Wolach, B ;

Dolfin, T ;

Regev, R ;

Gilboa, S ;

Schlesinger, M .

ACTA PAEDIATRICA, 1997, 86 (05) :523-527

[30] Biological variation in circulating levels of mannan-binding lectin (MBL) and MBL-Associated serine protease-2 and the influence of age, gender and physical exercise [J].

Ytting, H. ;

Christensen, I. J. ;

Thiel, S. ;

Jensenius, J. C. ;

Svendsen, M. N. ;

Nielsen, L. ;

Lottenburger, T. ;

Nielsen, H. J. .

SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2007, 66 (04) :458-464

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