Molecular Interaction Studies and Phytochemical Characterization of Mentha pulegium L. Constituents with Multiple Biological Utilities as Antioxidant, Antimicrobial, Anticancer and Anti-Hemolytic Agents

被引：36

作者：

Al-Rajhi, Aisha M. H. ^{[1
]}

Qanash, Husam ^{[2
,3
]}

Almuhayawi, Mohammed S. ^{[4
]}

Al Jaouni, Soad K. ^{[5
]}

Bakri, Marwah M. ^{[6
]}

Ganash, Magdah ^{[7
]}

Salama, Hanaa M. ^{[8
]}

Selim, Samy ^{[9
]}

Abdelghany, Tarek M. ^{[10
]}

机构：

[1] Princess Nourah Bint Abdulrahman Univ, Coll Sci, Dept Biol, POB 84428, Riyadh 11671, Saudi Arabia

[2] Univ Hail, Coll Appl Med Sci, Dept Med Lab Sci, Hail 55476, Saudi Arabia

[3] Univ Hail, Mol Diagnost & Personalized Therapeut Unit, Hail 55476, Saudi Arabia

[4] King Abdulaziz Univ, Fac Med, Dept Med Microbiol & Parasitol, Jeddah 21589, Saudi Arabia

[5] King Abdulaziz Univ, Fac Med, Dept Hematol Oncol, Yousef Abdulatif Jameel Sci Chair Prophet Med App, Jeddah 21589, Saudi Arabia

[6] Jazan Univ, Fac Sci, Biol Dept, Jazan 82817, Saudi Arabia

[7] King Abdulaziz Univ, Fac Sci, Biol Dept, Jeddah 21589, Saudi Arabia

[8] Port Said Univ, Fac Sci, Dept Chem, Port Said 42521, Egypt

[9] Jouf Univ, Coll Appl Med Sci, Dept Clin Lab Sci, Sakaka 72341, Saudi Arabia

[10] Al Azhar Univ, Fac Sci, Bot & Microbiol Dept, Cairo 11884, Egypt

来源：

MOLECULES | 2022年 / 27卷 / 15期

关键词：

Mentha pulegium; anticancer; anticoagulant; antimicrobial; antioxidant; IN-VITRO; CHEMICAL-COMPOSITION; PHENOLIC-COMPOUNDS; HPLC ANALYSIS; EXTRACTS; ACTIVATION; FLAVONOIDS; PULEGONE; SPICATA; ARREST;

D O I：

10.3390/molecules27154824

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Multiple biological functions of Mentha pulegium extract were evaluated in the current work. Phytochemical components of the M. pulegium extract were detected by Gas Chromatography-Mass Spectrometry (GC-MS) and High-performance liquid chromatography (HPLC). Moreover, M. pulegium extract was estimated for antioxidant potential by 2,2-Diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging, antimicrobial activity by well diffusion, and anticoagulant activity via prothrombin time (PT) and activated partial thromboplastin time (APTT). GC-MS analysis detected compounds including cholesterol margarate, stigmast-5-en-3-ol, 19-nor-4-androstenediol, androstan-17-one, pulegone-1,2-epoxide, isochiapin B, dotriacontane, hexadecanoic acid and neophytadiene. Chrysoeriol (15.36 mu g/mL) was followed by kaempferol (11.14 mu g/mL) and 7-OH flavone (10.14 mu g/mL), catechin (4.11 mu g/mL), hisperdin (3.05 mu g/mL), and luteolin (2.36 mu g/mL) were detected by HPLC as flavonoids, in addition to ferulic (13.19 mu g/mL), cinnamic (12.69 mu g/mL), caffeic (11.45 mu g/mL), pyrogallol (9.36 mu g/mL), p-coumaric (5.06 mu g/mL) and salicylic (4.17 mu g/mL) as phenolics. Antioxidant activity was detected with IC50 18 mu g/mL, hemolysis inhibition was recorded as 79.8% at 1000 mu g/mL, and PT and APTT were at 21.5 s and 49.5 s, respectively, at 50 mu g/mL of M. pulegium extract. The acute toxicity of M. pulegium extract was recorded against PC3 (IC50 97.99 mu g/mL) and MCF7 (IC50 80.21 mu g/mL). Antimicrobial activity of M. pulegium extract was documented against Bacillus subtilis, Escherichia coli, Pseudomonas aureus, Candida albicans, Pseudomonas aeruginosa, but not against black fungus Mucor circinelloides. Molecular docking was applied using MOE (Molecular Operating Environment) to explain the biological activity of neophytadiene, luteolin, chrysoeriol and kaempferol. These compounds could be suitable for the development of novel pharmacological agents for treatment of cancer and bacterial infections.

引用

页数：26