Impact of hydrolysis conditions on the detection of mannuronic to guluronic acid ratio in alginate and its derivatives

被引：35

作者：

Lu, Jiaojiao ^{[1
,2
]}

Yang, Hai ^{[3
,4
]}

Hao, Jie ^{[1
,2
]}

Wu, Chengling ^{[1
,2
]}

Liu, Li ^{[1
,2
]}

Xu, Naiyu ^{[1
,2
]}

Linhardt, Robert J. ^{[5
]}

Zhang, Zhenqing ^{[1
,2
]}

机构：

[1] Soochow Univ, Jiangsu Key Lab Translat Res & Therapy Neuropsych, Suzhou 215021, Jiangsu, Peoples R China

[2] Soochow Univ, Coll Pharmaceut Sci, Suzhou 215021, Jiangsu, Peoples R China

[3] Ocean Univ China, Dept Med & Pharm, Qingdao 266003, Peoples R China

[4] Qingdao Cent Hosp, Dept Pharm, Qingdao 266042, Shandong, Peoples R China

[5] Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

来源：

CARBOHYDRATE POLYMERS | 2015年 / 122卷

基金：

中国国家自然科学基金;

关键词：

Alginate; Ratio of mannuronic to guluronic acid (M/G); HPAEC-PAD; Hydrolysis conditions; ANION-EXCHANGE CHROMATOGRAPHY; SEQUENCE; OLIGOSACCHARIDES;

D O I：

10.1016/j.carbpol.2015.01.008

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Alginate is a linear and acidic polysaccharide, composed of (1 -> 4) linked beta-D-mannuronic acid (ManA) and alpha-L-guluronic acid (GulA). The ratio of ManA to GulA (M/G) is one of the most important factors for the application of alginate and its derivatives in various areas. In this work, a robust and accurate method was developed to analyze M/G using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The impact of hydrolysis conditions on the release patterns of ManA and GulA from alginate and its derivatives was investigated. The release patterns of ManA and GulA need to be considered separately to obtain an accurate M/G. Several hydrolysis conditions were established that released ManA and GulA completely and maintained these saccharide residues intact. The proper M/G of alginates from different sources and its derivatives could then be calculated by integration of the corresponding ManA and GulA peaks. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：180 / 188

页数：9

共 32 条

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