The Effects of Mentha pulegium L. Aqueous Extract and Nisin (Free and Nonoliposomes Forms) on Chemical, Biological, and Sensory Characteristics of Minced Silver Carp Fish (Hypophthalmichthys molitrix)

This study evaluated the effects of Mentha pulegium L. aqueous extract (AE) and nisin (free and nonoliposomes (N) forms) on chemical, biological, and sensory properties of minced silver carp fish during 12 days at 4 degrees C. The treatments included the following: control: without AE and nisin, treated samples with AE 1% and AE 2%, treated samples with N-AE 1% and N-AE 2%, and treated samples with nisin 1000 IU/g and N-nisin 1000 IU/g. The nanoliposomes were prepared with the mean sizes of 141-165 nm. Encapsulation efficiency (EE) was measured at 46.23-75.14%. The results showed that the nano aqueous extract (N-AE) group had the best effect on the microbial, chemical, and sensory properties in minced fish during storage. Also, AE was more effective on gram-negative bacteria (Escherichia coli). The values of minimum bactericidal concentration and minimum inhibitory concentration of gram-negative bacteria were higher than gram-positive bacteria.