Vascular-derived endothelial cell (EC) network prefabrication in three-dimensional (3D) tissue constructs before transplantation is useful for inducing functional anastomosis with the host vasculature. However, the clinical application of ECs is limited by cell isolation from the existing vasculature, because of the requirement for invasive biopsies and difficulty in obtaining a sufficient number of cells. Endothelial colony-forming cells (ECFCs), which are a subtype of endothelial progenitor cells in the blood, have a strong proliferative and vasculogenic potential. This study attempted to fabricate prevascular 3D cell-dense tissue constructs using cord blood-derived ECFCs and evaluate the in vivo angiogenic potential of these constructs. Human umbilical vascular endothelial cells (HUVECs) were also used in comparison with ECFCs, which were sandwiched between two human dermal-derived fibroblast (FB) sheets using a fibrin-coated cell-sheet manipulator. The inserted ECFCs in double-layered FB sheets were cultured for 3 days, resulting in the formation of network structures similar to those of HUVECs. Additionally, when ECFCs were sandwiched with three FB sheets, a lumen structure was found in the triple-layered cell-sheet constructs at 3 days after co-culture. These constructs containing ECFCs were transplanted into the subcutaneous tissue of immune-deficient rats. One week after transplantation, ECFC-lined functional microvessels containing rat erythrocytes were observed in the same manner as transplanted HUVEC-positive grafts. These results suggest that ECFCs might become an alternative cell source for fabricating a prevascular structure in 3D cell-dense tissue constructs for clinical application. Copyright (C) 2013 John Wiley & Sons, Ltd.
机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Asakawa, Nahoko
Shimizu, Tatsuya
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Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Shimizu, Tatsuya
Tsuda, Yukiko
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Univ Tokyo, Inst Ind Sci, Megro Ku, Tokyo 1538505, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Tsuda, Yukiko
Sekiya, Sachiko
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Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Sekiya, Sachiko
Sasagawa, Tadashi
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Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Sasagawa, Tadashi
Yamato, Masayuki
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Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Yamato, Masayuki
Fukai, Fumio
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h-index: 0
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Tokyo Univ Sci, Dept Pharm, Noda, Chiba 2788510, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Fukai, Fumio
Okano, Teruo
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Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
机构:
Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
Harvard Univ, MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USAHarvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
Au, Patrick
Tam, Joshua
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h-index: 0
机构:
Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
Harvard Univ, MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USAHarvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
Tam, Joshua
Fukumura, Dai
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h-index: 0
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Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USAHarvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
Fukumura, Dai
Jain, Rakesh K.
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h-index: 0
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Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USAHarvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Au, Patrick
Daheron, Laurence M.
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h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Ctr Regenerat Med, Sch Med, Cambridge, MA 02138 USA
Harvard Stem Cell Inst, Cambridge, MA USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Daheron, Laurence M.
Duda, Dan G.
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Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Duda, Dan G.
Cohen, Kenneth S.
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h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Ctr Regenerat Med, Sch Med, Cambridge, MA 02138 USA
Harvard Stem Cell Inst, Cambridge, MA USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Cohen, Kenneth S.
Tyrrell, James A.
论文数: 0引用数: 0
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Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Tyrrell, James A.
Lanning, Ryan M.
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h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Lanning, Ryan M.
Fukumura, Dai
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Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Fukumura, Dai
Scadden, David T.
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h-index: 0
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Harvard Univ, Massachusetts Gen Hosp, Ctr Regenerat Med, Sch Med, Cambridge, MA 02138 USA
Harvard Stem Cell Inst, Cambridge, MA USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Scadden, David T.
Jain, Rakesh K.
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Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
机构:
Indiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
Indiana Univ, Sch Med, Herman B Wells Ctr Pediat Res, Indianapolis, IN 46202 USAIndiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
Critser, P. J.
Kreger, S. T.
论文数: 0引用数: 0
h-index: 0
机构:
Purdue Univ, Sch Vet Med, Weldon Sch Biomed Engn, W Lafayette, IN 47906 USAIndiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
Kreger, S. T.
Voytik-Harbin, S. L.
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h-index: 0
机构:
Purdue Univ, Sch Vet Med, Weldon Sch Biomed Engn, W Lafayette, IN 47906 USA
Purdue Univ, Coll Engn, W Lafayette, IN 47906 USA
Purdue Univ, Dept Basic Med Sci, Sch Vet Med, W Lafayette, IN 47906 USAIndiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
Voytik-Harbin, S. L.
Yoder, M. C.
论文数: 0引用数: 0
h-index: 0
机构:
Indiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
Indiana Univ, Sch Med, Herman B Wells Ctr Pediat Res, Indianapolis, IN 46202 USA
Indiana Univ, Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USAIndiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, Japan
Elloumi-Hannachi, I.
Yamato, M.
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Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, Japan
Yamato, M.
Okano, T.
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Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, Japan
机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Asakawa, Nahoko
Shimizu, Tatsuya
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机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Shimizu, Tatsuya
Tsuda, Yukiko
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h-index: 0
机构:
Univ Tokyo, Inst Ind Sci, Megro Ku, Tokyo 1538505, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Tsuda, Yukiko
Sekiya, Sachiko
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h-index: 0
机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Sekiya, Sachiko
Sasagawa, Tadashi
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机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Sasagawa, Tadashi
Yamato, Masayuki
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h-index: 0
机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Yamato, Masayuki
Fukai, Fumio
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Sci, Dept Pharm, Noda, Chiba 2788510, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
Fukai, Fumio
Okano, Teruo
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, Shinjuku Ku, Tokyo 1628666, Japan
机构:
Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
Harvard Univ, MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USAHarvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
Au, Patrick
Tam, Joshua
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
Harvard Univ, MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USAHarvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
Tam, Joshua
Fukumura, Dai
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USAHarvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
Fukumura, Dai
Jain, Rakesh K.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USAHarvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Au, Patrick
Daheron, Laurence M.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Ctr Regenerat Med, Sch Med, Cambridge, MA 02138 USA
Harvard Stem Cell Inst, Cambridge, MA USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Daheron, Laurence M.
Duda, Dan G.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Duda, Dan G.
Cohen, Kenneth S.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Ctr Regenerat Med, Sch Med, Cambridge, MA 02138 USA
Harvard Stem Cell Inst, Cambridge, MA USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Cohen, Kenneth S.
Tyrrell, James A.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Tyrrell, James A.
Lanning, Ryan M.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Lanning, Ryan M.
Fukumura, Dai
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Fukumura, Dai
Scadden, David T.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Ctr Regenerat Med, Sch Med, Cambridge, MA 02138 USA
Harvard Stem Cell Inst, Cambridge, MA USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
Scadden, David T.
Jain, Rakesh K.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USAHarvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA
机构:
Indiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
Indiana Univ, Sch Med, Herman B Wells Ctr Pediat Res, Indianapolis, IN 46202 USAIndiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
Critser, P. J.
Kreger, S. T.
论文数: 0引用数: 0
h-index: 0
机构:
Purdue Univ, Sch Vet Med, Weldon Sch Biomed Engn, W Lafayette, IN 47906 USAIndiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
Kreger, S. T.
Voytik-Harbin, S. L.
论文数: 0引用数: 0
h-index: 0
机构:
Purdue Univ, Sch Vet Med, Weldon Sch Biomed Engn, W Lafayette, IN 47906 USA
Purdue Univ, Coll Engn, W Lafayette, IN 47906 USA
Purdue Univ, Dept Basic Med Sci, Sch Vet Med, W Lafayette, IN 47906 USAIndiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
Voytik-Harbin, S. L.
Yoder, M. C.
论文数: 0引用数: 0
h-index: 0
机构:
Indiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
Indiana Univ, Sch Med, Herman B Wells Ctr Pediat Res, Indianapolis, IN 46202 USA
Indiana Univ, Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USAIndiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA
机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, Japan
Elloumi-Hannachi, I.
Yamato, M.
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, Japan
Yamato, M.
Okano, T.
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, JapanTokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo, Japan