Drill-assisted genomic DNA extraction from Botrytis cinerea

被引：5

作者：

Gonzalez, Celedonio ^{[1
]}

Noda, Judith ^{[1
]}

Espino, Jose J. ^{[1
]}

Brito, Nelida ^{[1
]}

机构：

[1] Univ La Laguna, Dept Bioquim & Biol Mol, E-38206 Tenerife, Spain

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 11期

关键词：

Botrytis cinerea; DNA extraction; PCR; Southern-blot;

D O I：

10.1007/s10529-008-9790-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Current DNA extraction protocols for genomic DNA from Botrytis cinerea almost always start with mycelium that has been reduced to powder with liquid N-2 in a mortar, and this makes their application to a large number of samples slow and cumbersome. Here we present an adaptation of an existing method [Moller et al. (1992) Nucleic Acids Res 20: 6115-6116] for which the initial steps have been modified, including the homogenization of the fungus with sand and the aid of a common household drill. This method allows the processing of large number of samples in much shorter times and generates an average of 4 mu g DNA per sample, of sufficient quality for use in PCR and Southern blotting.

引用

页码：1989 / 1992

页数：4

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