Detection of GB virus-C hepatitis G virus RNA in serum by reverse transcription polymerase chain reaction

被引：0

作者：

Bhardwaj, B

Qian, KP

Detmer, J

Mizokami, M

Kolberg, JA

Urdea, MS

Schlauder, G

Linnen, JM

Kim, JP

Davis, GL

Lau, JYN

机构：

[1] UNIV FLORIDA,DEPT MED,SECT HEPATOBILIARY DIS,DIV GASTROENTEROL HEPATOL & NUTR,GAINESVILLE,FL 32610

[2] CHIRON CORP,EMERYVILLE,CA 94608

[3] NAGOYA CITY UNIV,DEPT MED 2,NAGOYA,AICHI,JAPAN

[4] ABBOTT LABS,VIRUS DISCOVERY GRP,EXPT BIOL RES,CHICAGO,IL

[5] GENELABS TECHNOL INC,REDWOOD CITY,CA

来源：

JOURNAL OF MEDICAL VIROLOGY | 1997年 / 52卷 / 01期

关键词：

GBV-C; HGV; HCV; RNA; PCR; hybridization; serum; sensitivity; specificity;

D O I：

10.1002/(SICI)1096-9071(199705)52:1<92::AID-JMV15>3.0.CO;2-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Three PCR methods based on the GB virus-C/hepatitis G virus (GBV-C/HGV) 5'UTR and NS3 genomic region were used for the detection of GBV-C/HGV RNA in serum of 62 patients with chronic hepatitis C virus (HCV) infection. Ten of 62 (16%) patients were found to have GBV-C/HGV RNA, which was confirmed by sequence analysis of the 5'UTR PCR amplicon. All methods appear to be specific, but methods based on the 5'UTR appear to be more sensitive. (C) 1997 Wiley-Liss, Inc.

引用

页码：92 / 96

页数：5

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