Glutathione-mediated release of functional plasmid DNA from positively charged quantum dots

被引：64

作者：

Li, Dan ^{[1
]}

Li, Gaiping ^{[1
,2
]}

Guo, Weiwei ^{[1
,2
]}

Li, Peicai ^{[1
,2
]}

Wang, Erkang ^{[1
]}

Wang, Jin ^{[3
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] SUNY Stony Brook, Dept Chem, Stony Brook, NY 11794 USA

来源：

BIOMATERIALS | 2008年 / 29卷 / 18期

基金：

中国国家自然科学基金;

关键词：

QDs; glutathione; plasmid DNA; controlled release;

D O I：

10.1016/j.biomaterials.2008.03.007

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

DNA was efficiently bound to water-soluble positively charged CdTe quantum dots (QDs) through complementary electrostatic interaction. These QDs-DNA complexes were disrupted and DNA was released by glutathione (GSH) at intracellular concentrations. Interestingly, there was almost no detectable DNA released by extracellular concentration of GSH. The formation of QDs-DNA complexes and GSH-mediated DNA release from the complexes were confirmed by dye displacement assay, electrophoretic mobility shift assay (EMSA), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) experiments. The released DNA retained transcriptional activity and expressed enhanced green fluorescent protein (EGFP) after being transfected into HEK 293 cells. The transfection efficiency measured by flow cytometry (FCM) was comparable with the positive control. The obvious difference of GSH concentration in nature between the intra- and extracellular environments as well as the GSH concentration-dependent triggered release suggests potential applications of these positively QDs in selective unpacking of payload in living cells in a visible manner. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2776 / 2782

页数：7

共 43 条

[1] Semiconductor clusters, nanocrystals, and quantum dots [J].

Alivisatos, AP .

SCIENCE, 1996, 271 (5251) :933-937

[2]

Anderson ME, 1998, CHEM-BIOL INTERACT, V112, P1

[3] One-pot synthesis of CdTe nanocrystals and shape control of luminescent CdTe-cystine nanocomposites [J].

Bao, HF ;

Wang, EK ;

Dong, SJ .

SMALL, 2006, 2 (04) :476-480

[4] A simple, high-resolution method for establishing DNA binding affinity and sequence selectivity [J].

Boger, DL ;

Fink, BE ;

Brunette, SR ;

Tse, WC ;

Hedrick, MP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (25) :5878-5891

[5] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[6] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[7] Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors [J].

Clapp, AR ;

Medintz, IL ;

Mauro, JM ;

Fisher, BR ;

Bawendi, MG ;

Mattoussi, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (01) :301-310

[8] Quantum dot-based multiplexed fluorescence resonance energy transfer [J].

Clapp, AR ;

Medintz, IL ;

Uyeda, HT ;

Fisher, BR ;

Goldman, ER ;

Bawendi, MG ;

Mattoussi, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (51) :18212-18221

[9] Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking [J].

Dahan, M ;

Lévi, S ;

Luccardini, C ;

Rostaing, P ;

Riveau, B ;

Triller, A .

SCIENCE, 2003, 302 (5644) :442-445

[10]

DEBERDT S, 1998, CLIN CHIM ACTA, V275, P175

← 1 2 3 4 5 →