Identification of a novel cell binding site of periostin involved in tumour growth

被引:56
作者
Orecchia, Paola [1 ]
Conte, Romana [1 ]
Balza, Enrica [2 ]
Castellani, Patrizia [2 ]
Borsi, Laura [2 ]
Zardi, Luciano [3 ]
Mingari, Maria Cristina [1 ,4 ]
Carnemolla, Barbara [1 ]
机构
[1] Ist Nazl Ric Canc, Immunol Lab, I-16132 Genoa, Italy
[2] Ist Nazl Ric Canc, Cell Biol Lab, I-16132 Genoa, Italy
[3] Ctr Biotecnol Avanzate, Lab Recombinant Therapeut Prot, Genoa, Italy
[4] Univ Genoa, Dept Expt Med, I-16126 Genoa, Italy
关键词
Matricellular protein; Extracellular matrix; Monoclonal antibody; Cell adhesion; FAS1; domain; Tumour growth; Angiogenesis; PANCREATIC-CANCER CELLS; FACTOR-BETA; EXTRACELLULAR-MATRIX; FASCICLIN-I; EXPRESSION; ANGIOGENESIS; PROTEIN; FIBRONECTIN; BETA-IG-H3; ADHESION;
D O I
10.1016/j.ejca.2011.04.026
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Introduction: Periostin (PN), a member of the fasciclin family of proteins, is a TGF-beta-induced extracellular matrix protein involved in cell survival, angiogenesis, invasion and metastasis. It is considered a potent angiogenic factor and a marker of tumour progression in many types of human cancer. Many different kinds of cells bind to PN by means of the integrins alpha v beta 3 and alpha v beta 5, but the periostin epitope recognised by these integrins is not formally demonstrated. The aim of our study was to identify which domain of PN could be involved in cell adhesion and its potential role in tumour growth. Methods: We generated the monoclonal antibody OC-20 (mAb OC-20) by hybridoma technology. Different PN recombinant fragments were used to characterise the periostin epitope recognised by the mAb OC-20 and to localise a new cell binding site of the protein. A murine model of human melanoma was used in the preclinical in vivo experiments. Results: We formally demonstrate that the periostin epitope recognised by OC-20 is a new binding site for the integrins alpha v beta 3 and alpha v beta 5, localised in the second FAS1 domain (FAS1-2) of the protein. Moreover the in vivo use of this antibody significantly inhibits tumour growth and angiogenesis. Conclusion: Our results show that the FAS1-2 domain of PN plays a role in tumour progression. Moreover this novel antibody may likewise prove to be very useful in clarifying the role of PN in angiogenesis and may contribute to the design of novel anti-angiogenesis drugs. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2221 / 2229
页数:9
相关论文
共 35 条
[1]   Periostin potently promotes metastatic growth of colon cancer by augmenting cell survival via the Akt/PKB pathway [J].
Bao, SD ;
Ouyang, G ;
Bai, XF ;
Huang, Z ;
Ma, CY ;
Liu, M ;
Shoo, R ;
Anderson, RM ;
Rich, JN ;
Wang, XF .
CANCER CELL, 2004, 5 (04) :329-339
[2]   Periostin promotes invasiveness and resistance of pancreatic cancer cells to hypoxia-induced cell death:: role of the β4 integrin and the PI3k pathway [J].
Baril, P. ;
Gangeswaran, R. ;
Mahon, P. C. ;
Caulee, K. ;
Kocher, H. M. ;
Harada, T. ;
Zhu, M. ;
Kalthoff, H. ;
Crnogorac-Jurcevic, T. ;
Lemoine, N. R. .
ONCOGENE, 2007, 26 (14) :2082-2094
[3]   Selective targeting of tumoral vasculature: Comparison of different formats of an antibody (L19) to the ED-B domain of fibronectin [J].
Borsi, L ;
Balza, E ;
Bestagno, M ;
Castellani, P ;
Carnemolla, B ;
Biro, A ;
Leprini, A ;
Sepulveda, J ;
Burrone, O ;
Neri, D ;
Zardi, L .
INTERNATIONAL JOURNAL OF CANCER, 2002, 102 (01) :75-85
[4]   THE FIBRONECTIN ISOFORM CONTAINING THE ED-B ONCOFETAL DOMAIN - A MARKER OF ANGIOGENESIS [J].
CASTELLANI, P ;
VIALE, G ;
DORCARATTO, A ;
NICOLO, G ;
KACZMAREK, J ;
QUERZE, G ;
ZARDI, L .
INTERNATIONAL JOURNAL OF CANCER, 1994, 59 (05) :612-618
[5]   Periostin creates a tumor-supportive microenvironment in the pancreas by sustaining fibrogenic stellate cell activity [J].
Erkan, Mert ;
Kleeff, Joerg ;
Gorbachevski, Andre ;
Reiser, Carolin ;
Mitkus, Tomas ;
Esposito, Irene ;
Giese, Thomas ;
Buechler, Markus W. ;
Giese, Nathalia A. ;
Friess, Helmut .
GASTROENTEROLOGY, 2007, 132 (04) :1447-1464
[6]   Periostin deposition in the stroma of invasive and intraductal neoplasms of the pancreas [J].
Fukushima, Noriyoshi ;
Kikuchi, Yoshinao ;
Nishiyama, Takashi ;
Kudo, Akira ;
Fukayama, Masashi .
MODERN PATHOLOGY, 2008, 21 (08) :1044-1053
[7]  
Gillan L, 2002, CANCER RES, V62, P5358
[8]   Functional role of periostin in development and wound repair: implications for connective tissue disease [J].
Hamilton, Douglas W. .
JOURNAL OF CELL COMMUNICATION AND SIGNALING, 2008, 2 (1-2) :9-17
[9]   Periostin shows increased evolutionary plasticity in its alternatively spliced region [J].
Hoersch, Sebastian ;
Andrade-Navarro, Miguel A. .
BMC EVOLUTIONARY BIOLOGY, 2010, 10
[10]   Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor β [J].
Horiuchi, K ;
Amizuka, N ;
Takeshita, S ;
Takamatsu, H ;
Katsuura, M ;
Ozawa, H ;
Toyama, Y ;
Bonewald, LF ;
Kudo, A .
JOURNAL OF BONE AND MINERAL RESEARCH, 1999, 14 (07) :1239-1249