Microfluidic Impedance Flow Cytometry Enabling High-Throughput Single-Cell Electrical Property Characterization

被引：117
|
作者：
Chen, Jian [1 ]
Xue, Chengcheng [1 ]
Zhao, Yang [1 ]
Chen, Deyong [1 ]
Wu, Min-Hsien [2 ]
Wang, Junbo [1 ]
机构：
[1] Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100190, Peoples R China
[2] Chang Gung Univ, Grad Inst Biochem & Biomed Engn, Taoyuan 333, Taiwan
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 05期
基金：
国家高技术研究发展计划(863计划); 中国国家自然科学基金;
关键词：
microfluidics; single-cell electrical property analysis; impedance flow cytometry; high throughput; RED-BLOOD-CELLS; MAXIMUM LENGTH SEQUENCES; PATCH-CLAMP TECHNIQUE; OF-CARE DIAGNOSTICS; BREAST-CANCER CELLS; MEMBRANE CAPACITANCE; CYTOPLASM CONDUCTIVITY; ON-CHIP; BIOPHYSICAL CHARACTERIZATION; PRACTICAL CONSIDERATIONS;
D O I：
10.3390/ijms16059804
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This article reviews recent developments in microfluidic impedance flow cytometry for high-throughput electrical property characterization of single cells. Four major perspectives of microfluidic impedance flow cytometry for single-cell characterization are included in this review: (1) early developments of microfluidic impedance flow cytometry for single-cell electrical property characterization; (2) microfluidic impedance flow cytometry with enhanced sensitivity; (3) microfluidic impedance and optical flow cytometry for single-cell analysis and (4) integrated point of care system based on microfluidic impedance flow cytometry. We examine the advantages and limitations of each technique and discuss future research opportunities from the perspectives of both technical innovation and clinical applications.
引用
收藏
页码：9804 / 9830
页数：27
相关论文
共 50 条
  • [1] Advance of microfluidic flow cytometry enabling high-throughput characterization of single-cell electrical and structural properties
    Huang, Xukun
    Chen, Xiao
    Tan, Huiwen
    Wang, Minruihong
    Li, Yimin
    Wei, Yuanchen
    Zhang, Jie
    Chen, Deyong
    Wang, Junbo
    Li, Yueying
    Chen, Jian
    CYTOMETRY PART A, 2024, 105 (02) : 139 - 145
  • [2] Developments of Conventional and Microfluidic Flow Cytometry Enabling High-Throughput Characterization of Single Cells
    Wang, Minruihong
    Liang, Hongyan
    Chen, Xiao
    Chen, Deyong
    Wang, Junbo
    Zhang, Yuan
    Chen, Jian
    BIOSENSORS-BASEL, 2022, 12 (07):
  • [3] Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
    Fan, Beiyuan
    Li, Xiufeng
    Chen, Deyong
    Peng, Hongshang
    Wang, Junbo
    Chen, Jian
    SENSORS, 2016, 16 (02):
  • [4] DEVELOPMENT OF A CROSSING CONSTRICTION CHANNEL BASED MICROFLUIDIC CYTOMETRY ENABLING THE HIGH-THROUGHPUT QUANTIFICATION OF SINGLE-CELL ELECTRICAL PHENOTYPES
    Zhang, Yi
    Zhao, Yang
    Chen, Deyong
    Wang, Ke
    Wei, Yuanchen
    Xu, Ying
    Wei, Hua
    Zhang, Guoqing
    Huang, Chengjun
    Wang, Junbo
    Chen, Jian
    2019 20TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS & EUROSENSORS XXXIII (TRANSDUCERS & EUROSENSORS XXXIII), 2019, : 1009 - 1012
  • [5] A Microfluidic Device Integrating Impedance Flow Cytometry and Electric Impedance Spectroscopy for High-Efficiency Single-Cell Electrical Property Measurement
    Feng, Yongxiang
    Huang, Liang
    Zhao, Peng
    Liang, Fei
    Wang, Wenhui
    ANALYTICAL CHEMISTRY, 2019, 91 (23) : 15204 - 15212
  • [6] Smartphone Imaging Flow Cytometry for High-Throughput Single-Cell Analysis
    Aslan, Mahmut Kamil
    Ding, Yun
    Stavrakis, Stavros
    Demello, Andrew J.
    ANALYTICAL CHEMISTRY, 2023, 95 (39) : 14526 - 14532
  • [7] Single-Cell Optical Absorbance Characterization With High-Throughput Microfluidic Microscopy
    Banoth, Earu
    Jagannadh, Veerendra Kalyan
    Gorthi, Sai Siva
    IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 2015, 22 (03) : 111 - 116
  • [8] Dynamic Microfluidic Cytometry for Single-Cell Cellomics: High-Throughput Probing Single-Cell-Resolution Signaling
    Chen, Peng
    Yan, Shuangqian
    Wang, Jie
    Guo, Yiran
    Dong, Yue
    Feng, Xiaojun
    Zeng, Xuemei
    Li, Yiwei
    Du, Wei
    Liu, Bi-Feng
    ANALYTICAL CHEMISTRY, 2019, 91 (02) : 1619 - 1626
  • [9] Single-cell microfluidic impedance cytometry: a review
    Sun, Tao
    Morgan, Hywel
    MICROFLUIDICS AND NANOFLUIDICS, 2010, 8 (04) : 423 - 443
  • [10] Single-cell microfluidic impedance cytometry: a review
    Tao Sun
    Hywel Morgan
    Microfluidics and Nanofluidics, 2010, 8 : 423 - 443
12345