Evaluation of Hydrogen Cyanide in the Blood of Fire Victims Based on the Kinetics of the Reaction with Ninhydrin

被引：1

作者：

Tabian, Daniel ^{[1
,2
]}

Bulgaru Iliescu, Diana ^{[2
]}

Diac, Madalina Maria ^{[2
]}

Badea, Mihaela ^{[1
]}

Toma, Sebastian Ionut ^{[1
]}

Drochioiu, Gabi ^{[3
]}

机构：

[1] Transilvania Univ Brasov, Fac Med, 56 Nicolae Balcescu St, Brasov 500019, Romania

[2] Grigore T Popa Univ Med & Pharm Iasi, Fac Med, 16 Univ St, Iasi 700115, Romania

[3] Alexandru Ioan Cuza Univ, Fac Chem, 11 Carol I, Iasi 700605, Romania

来源：

APPLIED SCIENCES-BASEL | 2022年 / 12卷 / 05期

关键词：

cyanide determination; forensic science; fire victims; blood samples; kinetics; ninhydrin; Conway vial; HEADSPACE GAS-CHROMATOGRAPHY; SPECTROPHOTOMETRIC DETERMINATION; SELECTIVE DETERMINATION; SMOKE-INHALATION; MICROEXTRACTION; THIOCYANATE; REAGENT;

D O I：

10.3390/app12052329

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An original kinetic spectrophotometric procedure was developed for the determination of hydrogen cyanide (HCN) in the whole blood of fire victims. Cyanide poisoning by smoke inhalation is common in forensic medicine, but the blood HCN of fire victims has not been studied in detail so far. In this research project, we developed a simple, fast, sensitive, and selective quantification method for both free and metabolized HCN based on the kinetics of cyanide reaction with ninhydrin. The method was linear in range, from 0.26 to 2.6 mu g mL(-1), with a coefficient of determination of r = 0.994. A high molar absorptivity of 4.95 x 10(5) L mol(-1) cm(-1) was calculated under the reaction conditions. The limit of quantification was 0.052 mu g mL(-1); the detection limit was 0.012 mu g mL(-1) and the standard error was +/- 2.7%. This micro method proved to be accurate, sensitive, and selective and has been successfully applied to the analysis of blood samples, allowing rapid monitoring of blood cyanide in several fire victims.

引用

页数：17