Mutations in the nucleotide binding pocket of MreB can alter cell curvature and polar morphology in Caulobacter

被引:41
作者
Dye, Natalie A. [1 ,2 ]
Pincus, Zachary [5 ]
Fisher, Isabelle C. [1 ,2 ]
Shapiro, Lucy [4 ]
Theriot, Julie A. [1 ,2 ,3 ]
机构
[1] Stanford Univ, Dept Biochem, Stanford, CA 94305 USA
[2] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA
[3] Stanford Univ, Dept Microbiol & Immunol, Stanford, CA 94305 USA
[4] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA
[5] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT USA
关键词
BACILLUS-SUBTILIS ACTIN; BACTERIAL CYTOSKELETON; WALL MORPHOGENESIS; PROTEINS MREB; SHAPE; FILAMENTS; DIVISION; HOMOLOG; LOCALIZATION; SEGREGATION;
D O I
10.1111/j.1365-2958.2011.07698.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The maintenance of cell shape in Caulobacter crescentus requires the essential gene mreB, which encodes a member of the actin superfamily and the target of the antibiotic, A22. We isolated 35 unique A22-resistant Caulobacter strains with single amino acid substitutions near the nucleotide binding site of MreB. Mutations that alter cell curvature and mislocalize the intermediate filament crescentin cluster on the back surface of MreB's structure. Another subset have variable cell widths, with wide cell bodies and actively growing thin extensions of the cell poles that concentrate fluorescent MreB. We found that the extent to which MreB localization is perturbed is linearly correlated with the development of pointed cell poles and variable cell widths. Further, we find that a mutation to glycine of two conserved aspartic acid residues that are important for nucleotide hydrolysis in other members of the actin superfamily abolishes robust midcell recruitment of MreB but supports a normal rate of growth. These mutant strains provide novel insight into how MreB's protein structure, subcellular localization, and activity contribute to its function in bacterial cell shape.
引用
收藏
页码:368 / 394
页数:27
相关论文
共 68 条
  • [1] The tubulin homologue FtsZ contributes to cell elongation by guiding cell wall precursor synthesis in Caulobacter crescentus
    Aaron, Michelle
    Charbon, Godefroid
    Lam, Hubert
    Schwarz, Heinz
    Vollmer, Waldemar
    Jacobs-Wagner, Christine
    [J]. MOLECULAR MICROBIOLOGY, 2007, 64 (04) : 938 - 952
  • [2] RodZ, a component of the bacterial core morphogenic apparatus
    Alyahya, S. Anisah
    Alexander, Roger
    Costa, Teresa
    Henriques, Adriano O.
    Emonet, Thierry
    Jacobs-Wagner, Christine
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (04) : 1239 - 1244
  • [3] [Anonymous], 1951, Activity Analysis of Production and Allocation-Proceedings of a Conference
  • [4] The bacterial cytoskeleton: An intermediate filament-like function
    Ausmees, N
    Kuhn, JR
    Jacobs-Wagner, C
    [J]. CELL, 2003, 115 (06) : 705 - 713
  • [5] A22 Disrupts the Bacterial Actin Cytoskeleton by Directly Binding and Inducing a Low-Affinity State in MreB
    Bean, G. J.
    Fllckinger, S. T.
    Westler, W. M.
    McCully, M. E.
    Sept, D.
    Weibel, D. B.
    Amann, K. J.
    [J]. BIOCHEMISTRY, 2009, 48 (22) : 4852 - 4857
  • [6] Polymerization properties of the Thermotoga maritima actin MreB:: Roles of temperature, nucleotides, and ions
    Bean, Greg J.
    Amann, Kurt J.
    [J]. BIOCHEMISTRY, 2008, 47 (02) : 826 - 835
  • [7] Belmont LD, 1999, J CELL SCI, V112, P1325
  • [8] Conditional lethality, division defects, membrane involution, and endocytosis in mre and mrd shape mutants of Escherichia coli
    Bendezu, Felipe O.
    de Boer, Piet A. J.
    [J]. JOURNAL OF BACTERIOLOGY, 2008, 190 (05) : 1792 - 1811
  • [9] RodZ (YfgA) is required for proper assembly of the MreB actin cytoskeleton and cell shape in E-coli
    Bendezu, Felipe O.
    Hale, Cynthia A.
    Bernhardt, Thomas G.
    de Boer, Piet A. J.
    [J]. EMBO JOURNAL, 2009, 28 (03) : 193 - 204
  • [10] AN ATPASE DOMAIN COMMON TO PROKARYOTIC CELL-CYCLE PROTEINS, SUGAR KINASES, ACTIN, AND HSP70 HEAT-SHOCK PROTEINS
    BORK, P
    SANDER, C
    VALENCIA, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) : 7290 - 7294