Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy

被引：57

作者：

Park, HyunJoo ^{[1
]}

Hong, Sung-Hee ^{[2
]}

Kim, Kyoohyun ^{[1
]}

Cho, Shin-Hyeong ^{[2
]}

Lee, Won-Ja ^{[2
]}

Kim, Youngchan ^{[1
]}

Lee, Sang-Eun ^{[2
]}

Park, YongKeun ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Phys, Taejon 305701, South Korea

[2] Korea Ctr Dis Control & Prevent, Korea Natl Inst Hlth, Div Malaria & Parasit Dis, Chungbuk 363951, South Korea

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

新加坡国家研究基金会;

关键词：

DIFFRACTION OPTICAL TOMOGRAPHY; RECENT INSIGHTS; PHASE; DYNAMICS; VOLUME; PATHOPHYSIOLOGY; ERYTHROCYTES; HEMOGLOBIN; MALARIA; DISEASE;

D O I：

10.1038/srep10827

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Babesia microti causes "emergency" human babesiosis. However, little is known about the alterations in B. microti invaded red blood cells (Bm-RBCs) at the individual cell level. Through quantitative phase imaging techniques based on laser interferometry, we present the simultaneous measurements of structural, chemical, and mechanical modifications in individual mouse Bm-RBCs. 3-D refractive index maps of individual RBCs and in situ parasite vacuoles are imaged, from which total contents and concentration of dry mass are also precisely quantified. In addition, we examine the dynamic membrane fluctuation of Bm-RBCs, which provide information on cell membrane deformability.

引用

页数：11

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