New practical technique for protein crystallization with floating and stirring methods

被引：18
作者：
Adachi, H
Matsumura, H
Takano, K
Niino, A
Inoue, T
Yoshimura, M
Mori, Y
Sasaki, T
机构：
[1] Osaka Univ, Dept Elect Engn, Suita, Osaka 5650871, Japan
[2] Osaka Univ, SOSHO Project, Suita, Osaka 5650871, Japan
[3] Osaka Univ, Venture Business Lab, Suita, Osaka 5650871, Japan
[4] Osaka Univ, Dept Chem Mat, Suita, Osaka 5650871, Japan
[5] Osaka Univ, Dept Mat & Life Sci, Suita, Osaka 5650871, Japan
[6] PRESTO, JST, Suita, Osaka 5650871, Japan
来源：
JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS | 2003年 / 42卷 / 10A期
关键词：
protein crystal growth; stirring solution; microscale; vapor diffusion technique; X-ray diffraction;
D O I：
10.1143/JJAP.42.L1161
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
We previously developed an effective method for growing large, high-quality protein crystals. The method, which we call the floating and stirring technique (FAST), enables us to grow crystals on an insoluble and very dense liquid without contacting the growth vessel, and to stir a protein solution mildly. Our newly designed technique, Micro-FAST, makes FAST suitable for practical use. Micro-FAST enables us to reduce the sample volume and is applied to the vapor diffusion technique. Crystals grown by Micro-FAST demonstrated excellent X-ray diffraction with high resolution.
引用
收藏
页码：L1161 / L1163
页数：3
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