Repositioning of Aurora B Promoted by Chiasmata Ensures Sister Chromatid Mono-Orientation in Meiosis I

被引:56
作者
Sakuno, Takeshi [1 ]
Tanaka, Koichi [1 ]
Hauf, Silke [2 ]
Watanabe, Yoshinori [1 ]
机构
[1] Univ Tokyo, Lab Chromosome Dynam, Inst Mol & Cellular Biosci, Tokyo 1130032, Japan
[2] Max Planck Gesell, Friedrich Miescher Lab, D-72076 Tubingen, Germany
关键词
1ST MEIOTIC DIVISION; PROTEIN PHOSPHATASE 2A; SCHIZOSACCHAROMYCES-POMBE; CHROMOSOME SEGREGATION; FISSION YEAST; MONOPOLIN COMPLEX; RECOMBINATION; COHESION; SPINDLE; KINETOCHORES;
D O I
10.1016/j.devcel.2011.08.012
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
During meiosis I, kinetochores of sister chromatids are juxtaposed or fused and mono-orient, while homologous chromosomes that are paired by chiasmata (bivalents) have to biorient. In the absence of chiasmata, biorientation of sister chromatids (univalents), which carries a risk of aneuploidy, has been occasionally detected in several species, including humans. We show in fission yeast that biorientation of fused sister kinetochores predominates during early prometaphase I. Without chiasmata, this undesirable biorientation of univalents persists and eventually evades the spindle assembly checkpoint, provoking abnormal anaphase. When univalents are connected by chiasmata or by an artificial tether, this erroneous attachment is converted to monopolar attachment and stabilized. This stabilization is apparently achieved by a chromosome configuration that brings kinetochores to the outer edge of the bivalent, while bringing Aurora B, a destabilizer of kinetochore-microtubule attachment, inward. Our results elucidate how chiasmata favor biorientation of bivalents over that of univalents at meiosis I.
引用
收藏
页码:534 / 545
页数:12
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