Imaging of single molecules in live cells

被引:4
|
作者
Izeddin, Ignacio [1 ,2 ]
Darzacq, Xavier [1 ]
Dahan, Maxime [1 ,2 ]
机构
[1] Ecole Normale Super, Inst Biol, F-75005 Paris, France
[2] Dept Phys, Lab Kastler Brossel, F-75005 Paris, France
来源
M S-MEDECINE SCIENCES | 2011年 / 27卷 / 05期
关键词
FLUORESCENCE MICROSCOPY;
D O I
10.1051/medsci/2011275022
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Imaging of single molecules in live cells Progress in optical microscopy, combined to the emergence of new fluorescent probes and advanced instrumentation, now permits the imaging of single molecules in fixed and live cells. This extreme detection sensitivity has opened new modalities in cellular imaging. On the one hand, optical images with an unprecedented resolution in the 10-50 nm range, well below the diffraction limit of light, can be recorded. These super-resolution images give new insights into the properties of cellular structures. On the other hand, proteins, either in the membrane or intracellular, can be tracked in live cells and in physiological conditions. Their individual trajectories provide invaluable information on the molecular interactions that control their dynamics and their spatial organization. Single molecule imaging is rapidly becoming a unique tool to understand the biochemical and biophysical processes that determine the properties of molecular assemblies in a cellular context.
引用
收藏
页码:547 / 552
页数:6
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