Characterization of bovine atrial angiotensin-converting enzyme

被引：3
作者：
Garats, EV
Nikolskaya, II
Binevski, PV
Pozdnev, VF
Kost, OA [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Sch Chem, Moscow 119899, Russia
[2] Russian Acad Med Sci, Orekhovich Inst Biomed Chem, Moscow 119832, Russia
来源：
BIOCHEMISTRY-MOSCOW | 2001年 / 66卷 / 04期
基金：
俄罗斯基础研究基金会;
关键词：
angiotensin converting enzyme; tissue specificity; bovine atrium; catalytic properties;
D O I：
10.1023/A:1010205614096
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bovine atrial angiotensin-converting enzyme (ACE) was purified to electrophoretic homogeneity. The purification procedure included ion-exchange chromatography on DEAE-Toyopearl 650M, affinity chromatography on lisinopril-agarose and eel filtration on Sephadex G-100. The bovine atrial ACE exhibited similar sensitivities to inhibition by lisinopril and captopril as lung AC E (the K,values for the atrial and lung enzymes differed insignificantly). However, the kinetic parameters of hydrolysis of some synthetic tripeptide substrates (FA-Phe-Gly-Gly, FA-Phe-Phe-Arg, Cbz-Phe-His-Leu, Hip-His-Leu) catalyzed by bovine atrial and lung ACE varied to a greater extent. The enzymes were also characterized by some differences in activation by chloride, nit rate, and sulfate an ions. These data support the hypothesis of tissue specificity of ACEs.
引用
收藏
页码：429 / 434
页数：6
相关论文
共 50 条
  • [21] Novel findings: Expression of angiotensin-converting enzyme and angiotensin-converting enzyme 2 in thoracic aortic dissection and aneurysm
    Li, Yang
    Hu, Jia
    Qian, Hong
    Gu, Jun
    Meng, Wei
    Zhang, Er-yong
    [J]. JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM, 2015, 16 (04) : 1130 - 1134
  • [22] Effect of angiotensin-converting enzyme gene insertion/deletion polymorphism and angiotensin-converting enzyme inhibition on erythropoiesis in patients on haemodialysis
    Kiss, Zoltan
    Ambrus, Csaba
    Kulcsar, Imre
    Szegedi, Janos
    Kiss, Istvan
    [J]. JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM, 2015, 16 (04) : 1021 - 1027
  • [23] Angiotensin-Converting Enzyme and Angiotensin-Converting Enzyme 2 Are Involved in Sinoaortic Denervation-Induced Cardiovascular Hypertrophy in Rats
    Zhang, Li-Chao
    Li, Zhen-Zhen
    Yu, Yong-Sheng
    Wang, Zhi-Bin
    Wei, Xin
    Su, Ding-Feng
    Li, Ling
    [J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2011, 34 (09) : 1398 - 1403
  • [24] The polymorphisms in angiotensin-converting enzyme and angiotensin-converting enzyme 2 are not associated with thoracic aortic diseases and coronary heart disease
    Li, Yang
    Zhang, Er Yong
    Wu, Zhong
    [J]. PROCEEDINGS OF SINGAPORE HEALTHCARE, 2016, 25 (03) : 148 - 151
  • [25] Intrarenal Distributions and Changes of Angiotensin-Converting Enzyme and Angiotensin-Converting Enzyme 2 in Feline and Canine Chronic Kidney Disease
    Mitani, Sawane
    Yabuki, Akira
    Sawa, Mariko
    Chang, Hye-Sook
    Yamato, Osamu
    [J]. JOURNAL OF VETERINARY MEDICAL SCIENCE, 2014, 76 (01) : 45 - 50
  • [26] Imbalance Between Pulmonary Angiotensin-Converting Enzyme and Angiotensin-Converting Enzyme 2 Activity in Acute Respiratory Distress Syndrome
    Wosten-van Asperen, Roelie M.
    Bos, Albert P.
    Bem, Reinout A.
    Dierdorp, Barbara S.
    Dekker, Tamara
    van Goor, Harry
    Kamilic, Jelena
    van der Loos, Chris M.
    van den Berg, Elske
    Bruijn, Martijn
    van Woensel, Job B.
    Lutter, Rene
    [J]. PEDIATRIC CRITICAL CARE MEDICINE, 2013, 14 (09) : E438 - E441
  • [27] Inhibitor analysis of angiotensin I-converting and kinin-degrading activities of bovine lung and testicular angiotensin-converting enzyme
    Grinshtein, SV
    Binevski, PV
    Gomazkov, OA
    Pozdnev, VP
    Nikolskaya, II
    Kost, OA
    [J]. BIOCHEMISTRY-MOSCOW, 1999, 64 (08) : 938 - 944
  • [28] Estrogen regulation of angiotensin-converting enzyme mRNA
    Gallagher, PE
    Li, P
    Lenhart, JR
    Chappell, MC
    Brosnihan, KB
    [J]. HYPERTENSION, 1999, 33 (01) : 323 - 328
  • [29] Novel roles of the renal angiotensin-converting enzyme
    Giani, Jorge F.
    Veiras, Luciana C.
    Shen, Justin Z. Y.
    Bernstein, Ellen A.
    Cao, DuoYao
    Okwan-Duodu, Derick
    Khan, Zakir
    Gonzalez-Villalobos, Romer A.
    Bernstein, Kenneth E.
    [J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2021, 529
  • [30] Some aspects of the angiotensin-converting enzyme for leukemias
    Orlova M.A.
    Orlov A.P.
    [J]. Russian Chemical Bulletin, 2016, 65 (5) : 1380 - 1382
12345