Phylogenomic analysis for Campylobacter fetus ocurring in Argentina

被引:5
作者
Daniel Farace, Pablo [1 ]
Matias Irazoqui, Jose [2 ]
Graciela Morsella, Claudia [3 ]
Agustin Garcia, Juan [3 ]
Alejandra Mendez, Maria [3 ]
Alberto Paolicchi, Fernando [3 ]
Fernando Amadio, Ariel [2 ]
Karina Gioffre, Andrea [1 ]
机构
[1] Inst Nacl Tecnol Agr, Inst Agrobiotecnol & Biol Mol, CONICET, IABIMO INTA, Hurlingham, Buenos Aires, Argentina
[2] Consejo Nacl Invest Cient & Tecn, Estn Expt Agr INTA, Rafaela, Santa Fe, Argentina
[3] Univ Nacl Mar del Plata, Unidad Integrada INTA, Grp Sanidad Anim, Lab Bacteriol, Balcarce, Buenos Aires, Argentina
关键词
Campylobacter fetus; multi-locus sequence typing; pangenome; phylogenomics; venereal disease; BOVINE ABORTION; PCR; IDENTIFICATION; INCONSISTENCY;
D O I
10.14202/vetworld.2021.1165-1179
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Background and Aim: Campylobacter fetus is one of the most important pathogens that severely affects livestock industry worldwide. C. fetus mediated bovine genital campylobacteriosis infection in cattle has been associated with significant economic losses in livestock production in the Pampas region, the most productive area of Argentina The present study aimed to establish the genomic relationships between C. fetus strains, isolated from the Pampas region, at local and global levels. The study also explored the utility of multi-locus sequence typing (MLST) as a typing technique for C. fetus. Materials and Methods: For pangenome and phylogenetic analysis, whole genome sequences for 34 C. fetus strains, isolated from cattle in Argentina were downloaded from GenBank. A local maximum likelihood (ML) tree was constructed and linked to a Microreact project. In silico analysis based on MLST was used to obtain information regarding sequence type (ST) for each strain. For global phylogenetic analysis, a core genome ML-tree was constructed using genomic dataset for 265 C. fetus strains, isolated from various sources obtained from 20 countries. Results: The local core genome phylogenetic tree analysis described the presence of two major clusters (A and B) and one minor cluster (C). The occurrence of 82% of the strains in these three clusters suggested a clonal population structure for C. fetus. The MLST analysis for the local strains revealed that 31 strains were ST4 type and one strain was ST5 type. In addition, a new variant was identified that was assigned a novel ST, ST70. In the present case, ST4 was homogenously distributed across all the regions and clusters. The global analysis showed that most of the local strains clustered in the phylogenetic groups that comprised exclusively of the strains isolated from Argentina. Interestingly, three strains showed a close genetic relationship with bovine strains obtained from Uruguay and Brazil. The ST5 strain grouped in a distant cluster, with strains obtained from different sources from various geographic locations worldwide. Two local strains clustered in a phylogenetic group comprising intercontinental Campylobacter fetus venerealis strains. Conclusion: The results of the study suggested active movement of animals, probably due to economic trade between different regions of the country as well as with neighboring countries. MLST results were partially concordant with phylogenetic analysis. Thus, this method did not qualify as a reliable subtyping method to assess C. fetus diversity in Argentina. The present study provided a basic platform to conduct future research on C. fetus, both at local and international levels.
引用
收藏
页码:1165 / 1179
页数:15
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[41]   Whole genome sequence analysis indicates recent diversification of mammal-associated Campylobacter fetus and implicates a genetic factor associated with H2S production [J].
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[43]   Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus [J].
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[46]   Pericarditis caused by Campylobacter fetus subspecies fetus associated with ingestion of raw beef liver [J].
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[47]   Meningoencephalitis Caused by a Campylobacter fetus in a Patient with Chronic Alcoholism [J].
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[50]   Identification of Campylobacter fetus by fluorescence in situ hybridization (FISH) [J].
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Gross, Uwe ;
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Tomaso, Herbert ;
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