What is the role of thermodynamics on protein stability?

被引：45

作者：

Gummadi, SN ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem Engn, Madras 600036, Tamil Nadu, India

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2003年 / 8卷 / 01期

关键词：

protein synthesis; stability; protein folding; thermodynamics; entropy; enthalpy;

D O I：

10.1007/BF02932892

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The most challenging and emerging field of biotechnology is the tailoring of proteins to attain the desired characteristic properties. In order to increase the stability of proteins and to study the function of proteins, the mechanism by which proteins fold and unfold should be known. It has been debated for a long time how exactly the linear form of a protein is converted into a stable 3-dimensional structure. The literature showed that many theories support the fact that protein folding is a thermodynamically controlled process. It is also possible to predict the mechanism of protein deactivation and stability to an extent from thermodynamic studies. This article reviewed various theories that have been proposed to explain the process of protein folding after its biosynthesis in ribosomes. The theories of the determination of the thermodynamic properties and the interpretation of thermodynamic data of protein stability are also discussed in this article.

引用

页码：9 / 18

页数：10

共 83 条

[61] HOW DOES PROTEIN-SYNTHESIS GIVE RISE TO THE 3D-STRUCTURE [J].

PTITSYN, OB .

FEBS LETTERS, 1991, 285 (02) :176-181

[62] Thermodynamic studies of substrate binding and spin transitions in human cytochrome P-450 3A4 expressed in yeast microsomes [J].

Renaud, JP ;

Davydov, DR ;

Heirwegh, KPM ;

Mansuy, D ;

Hoa, GHB .

BIOCHEMICAL JOURNAL, 1996, 319 :675-681

[63] Enzymes and the supramolecular organization of the living cell. Information transfer within supramolecular edifices and imprinting effects [J].

Ricard, J ;

Gontero, B ;

Avilan, L ;

Lebreton, S .

CELLULAR AND MOLECULAR LIFE SCIENCES, 1998, 54 (11) :1231-1248

[64] Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli [J].

Rizzitello, AE ;

Harper, JR ;

Silhavy, TJ .

JOURNAL OF BACTERIOLOGY, 2001, 183 (23) :6794-6800

[65]

Sadana A, 1995, BIOCATALYSIS FUNDAME

[66] Templating peptide folding on the surface of a micelle:: Nucleating the formation of a β-hairpin [J].

Searle, MS ;

Jourdan, M .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2000, 10 (10) :1139-1142

[67] Protein synthesis - The perks of balancing glucose [J].

Sonenberg, N ;

Newgard, CB .

SCIENCE, 2001, 293 (5531) :818-819

[68]

Soti C, 2002, CELL STRESS CHAPERON, V7, P186, DOI 10.1379/1466-1268(2002)007<0186:CCOA>2.0.CO

[69]

[70] Protein misfolding and disease; protein refolding and therapy [J].

Soto, C .

FEBS LETTERS, 2001, 498 (2-3) :204-207

← 1 2 3 4 5 6 7 8 9 →