A simple, efficient tool for assessment of mice after unilateral cortex injury

被引：35

作者：

Shelton, Shirley B. ^{[1
]}

Pettigrew, David B. ^{[1
]}

Hermann, Alison D. ^{[1
]}

Zhou, Weidong ^{[1
]}

Sullivan, Patrick M. ^{[2
]}

Crutcher, Keith A. ^{[1
]}

Strauss, Kenneth I. ^{[1
]}

机构：

[1] Univ Cincinnati, Div Res, Dept Neurosurg, Cincinnati, OH 45267 USA

[2] Duke Univ, Durham VA Med Ctr, Div Geriatr, Dept Med, Durham, NC 27705 USA

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2008年 / 168卷 / 02期

关键词：

apolipoprotein E; targeted-replacernent; beam walk; foot fault; water maze;

D O I：

10.1016/j.jneumeth.2007.11.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A refined battery of neurological tests, SNAP (Simple Neuroassessment of Asymmetric Impairment), was developed and validated to efficiently assess neurological deficits induced in a mouse model of traumatic brain injury. Four to 7-month old mice were subjected to unilateral controlled cortical impact or sham injury (craniectomy only). Several behavioral tests (SNAP, beam walk, foot fault, and water maze) were used to assess functional deficits. SNAP was unique among these in that it required no expensive equipment and was performed in less than 5 min per mouse. SNAP demonstrated a high level of sensitivity and specificity as determined by receiver-operator characteristics curve analysis. Interrater reliability was good, as determined by Cohen's Kappa method and by comparing the sensitivity and specificity across various raters. SNAP detected deficits in proprioception, visual fields, and motor strength in brain-injured mice at 3 days, and was sensitive enough to detect magnitude and recovery of injury. The contribution of individual battery components changed as a function of time after injury, however, each was important to the overall SNAP score. SNAP provided a sensitive, reliable, time-efficient and cost-effective means of assessing neurological deficits in mice after unilateral brain injury. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：431 / 442

页数：12