Molecular Epidemiology of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus in Wild, Captive and Laboratory Rats: Effect of Habitat on the Nasal S. aureus Population

被引：20

作者：

Raafat, Dina ^{[1
,2
]}

Mrochen, Daniel M. ^{[1
]}

Al'Sholui, Fawaz ^{[1
]}

Heuser, Elisa ^{[3
]}

Ryll, Rene ^{[3
]}

Pritchett-Corning, Kathleen R. ^{[4
,21
]}

Jacob, Jens ^{[5
]}

Walther, Bernd ^{[5
]}

Matuschka, Franz-Rainer ^{[6
]}

Richter, Dania ^{[7
]}

Westerhues, Uta ^{[8
]}

Pikula, Jiri ^{[9
,10
]}

van den Brandt, Jens ^{[11
,12
]}

Nicklas, Werner ^{[13
]}

Monecke, Stefan ^{[14
,15
]}

Strommenger, Birgit ^{[16
]}

Van Alen, Sarah ^{[17
,20
]}

Becker, Karsten ^{[17
,18
]}

Ulrich, Rainer G. ^{[3
,19
]}

Holtfreter, Silva ^{[1
]}

机构：

[1] Univ Med Greifswald, Dept Immunol, D-17475 Greifswald, Germany

[2] Alexandria Univ, Dept Microbiol & Immunol, Fac Pharm, Alexandria 21521, Egypt

[3] Friedrich Loeffler Inst, Inst Novel & Emerging Infect Dis, Fed Res Inst Anim Hlth, D-17493 Greifswald, Germany

[4] Charles River Labs, Res & Profess Serv, Wilmington, MA 01887 USA

[5] Julius Kuhn Inst, Fed Res Ctr Cultivated Plants, Inst Plant Protect Hort & Forestry, Vertebrate Res, D-48161 Munster, Germany

[6] Univ Potsdam, Outpatient Clin, D-14469 Potsdam, Germany

[7] Tech Univ Carolo Wilhelmina Braunschweig, Inst Geoecol Landscape Ecol & Environm Syst Anal, D-38106 Braunschweig, Germany

[8] Opel Hess Zoostiftung, D-61476 Kronberg Im Taunus, Germany

[9] Univ Vet & Pharmaceut Sci Brno, Dept Ecol & Dis Game Fish & Bees, Brno 61242, Czech Republic

[10] Univ Vet & Pharmaceut Sci Brno, CEITEC Cent European Inst Technol, Brno 61242, Czech Republic

[11] Univ Med Greifswald, Cent Core, D-17475 Greifswald, Germany

[12] Univ Med Greifswald, Res Facil Lab Anim, D-17475 Greifswald, Germany

[13] German Canc Res Ctr, Microbiol Diagnost, D-69120 Heidelberg, Germany

[14] Leibniz Inst Photon Technol IPHT, D-07745 Jena, Germany

[15] Tech Univ Dresden, Inst Med Microbiol & Hyg, D-01307 Dresden, Germany

[16] Robert Koch Inst, Wernigerode Branch, Natl Reference Ctr Staphylococci & Enterococci, D-38855 Wernigerode, Germany

[17] Univ Hosp Munster, Inst Med Microbiol, D-48149 Munster, Germany

[18] Univ Med Greifswald, Friedrich Loeffler Inst Med Microbiol, D-17475 Greifswald, Germany

[19] German Ctr Infect Res DZIF, Partner Site Hamburg Lubeck Borstel Insel Riems, D-17493 Greifswald, Germany

[20] Grunenthal GmbH, D-52224 Stolberg, Germany

[21] Harvard Univ, Fac Arts & Sci, Off Anim Resources, Cambridge, MA 02138 USA

来源：

TOXINS | 2020年 / 12卷 / 02期

关键词：

Staphylococcus aureus; rat; clonal complex; host adaptation; livestock; laboratory; coagulation; immune evasion cluster; habitat; epidemiology; CHEMOTAXIS INHIBITORY PROTEIN; RATTUS-NORVEGICUS; IMMUNE EVASION; ANTIBIOTIC-RESISTANCE; COMPLEMENT INHIBITOR; GENE-CLUSTER; NORWAY RATS; MODEL; SUPERANTIGENS; INFECTION;

D O I：

10.3390/toxins12020080

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Rats are a reservoir of human- and livestock-associated methicillin-resistant Staphylococcus aureus (MRSA). However, the composition of the natural S. aureus population in wild and laboratory rats is largely unknown. Here, 144 nasal S. aureus isolates from free-living wild rats, captive wild rats and laboratory rats were genotyped and profiled for antibiotic resistances and human-specific virulence genes. The nasal S. aureus carriage rate was higher among wild rats (23.4%) than laboratory rats (12.3%). Free-living wild rats were primarily colonized with isolates of clonal complex (CC) 49 and CC130 and maintained these strains even in husbandry. Moreover, upon livestock contact, CC398 isolates were acquired. In contrast, laboratory rats were colonized with many different S. aureus lineages-many of which are commonly found in humans. Five captive wild rats were colonized with CC398-MRSA. Moreover, a single CC30-MRSA and two CC130-MRSA were detected in free-living or captive wild rats. Rat-derived S. aureus isolates rarely harbored the phage-carried immune evasion gene cluster or superantigen genes, suggesting long-term adaptation to their host. Taken together, our study revealed a natural S. aureus population in wild rats, as well as a colonization pressure on wild and laboratory rats by exposure to livestock- and human-associated S. aureus, respectively.

引用

页数：22

共 103 条

[81] Staphylococcus aureus host specificity:: comparative genomics of human versus animal isolates by multi-strain microarray [J].