Effect of the coffee ingredient cafestol on head and neck squamous cell carcinoma cell lines

被引：12

作者：

Kotowski, Ulana ^{[1
]}

Heiduschka, Gregor ^{[1
]}

Seemann, Rudolf ^{[2
]}

Eckl-Dorna, Julia ^{[1
]}

Schmid, Rainer ^{[3
]}

Kranebitter, Veronika ^{[1
]}

Stanisz, Isabella ^{[1
]}

Brunner, Markus ^{[1
]}

Lill, Claudia ^{[1
]}

Thurnher, Dietmar ^{[1
]}

机构：

[1] Med Univ Vienna, Dept Otorhinolaryngol Head & Neck Surg, A-1090 Vienna, Austria

[2] Med Univ Vienna, Dept Cranio Maxillofacial & Oral Surg, A-1090 Vienna, Austria

[3] Med Univ Vienna, Dept Radiotherapy, A-1090 Vienna, Austria

来源：

STRAHLENTHERAPIE UND ONKOLOGIE | 2015年 / 191卷 / 06期

基金：

奥地利科学基金会;

关键词：

Phytochemicals; Irradiation; Head and neck cancer; Cytotoxins; Cisplatin; NATURAL-PRODUCTS; DRUG DISCOVERY; CANCER; COMBINATION; DITERPENES; KAHWEOL;

D O I：

10.1007/s00066-014-0807-x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background and purpose Cafestol is a diterpene molecule found in coffee beans and has anticarcinogenic properties. The aim of the study was to examine the effects of cafestol in head and neck squamous cell carcinoma (HNSCC) cells. Materials and methods Three HNSCC cell lines (SCC25, CAL27 and FaDu) were treated with increasing doses of cafestol. Then combination experiments with cisplatin and irradiation were carried out. Drug interactions and possible synergy were calculated using the combination index analysis. Clonogenic assays were performed after irradiation with 2, 4, 6 and 8 Gy, respectively, and the rate of apoptosis was measured with flow cytometry. Results Treatment of HNSCC cells with cafestol leads to a dose-dependent reduction of cell viability and to induction of apoptosis. Combination with irradiation shows a reduction of clonogenic survival compared to each treatment method alone. In two of the cell lines a significant additive effect was observed. Conclusion Cafestol is a naturally occurring effective compound with growth-inhibiting properties in head and neck cancer cells. Moreover, it leads to a significant inhibition of colony formation.

引用

页码：511 / 517

页数：7

共 22 条

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