Mass-based metabolomic analysis of soybean sprouts during germination

被引：86

作者：

Gu, Eun-Ji ^{[1
]}

Kim, Dong Wook ^{[1
]}

Jang, Gwang-Ju ^{[1
]}

Song, Seong Hwa ^{[1
]}

Lee, Jae-In ^{[1
]}

Lee, Sang Bong ^{[1
]}

Kim, Bo-Min ^{[1
]}

Cho, Yeongrae ^{[1
]}

Lee, Hyeon-Jeong ^{[1
]}

Kim, Hyun-Jin ^{[1
,2
,3
]}

机构：

[1] Gyeongsang Natl Univ, Div Appl Life Sci Plus BK21, 501 Jinjudaero, Jinju, Gyeongsang, South Korea

[2] Gyeongsang Natl Univ, Dept Food Sci & Technol, 501 Jinjudaero, Jinju, Gyeongsang, South Korea

[3] Gyeongsang Natl Univ, Inst Agr & Life Sci, 501 Jinjudaero, Jinju, Gyeongsang, South Korea

来源：

FOOD CHEMISTRY | 2017年 / 217卷

基金：

新加坡国家研究基金会;

关键词：

Soybean sprouts; Germination; Metabolomics; GC-MS; LC-MS; D-CHIRO-INOSITOL; GLYCINE-MAX; GAS-CHROMATOGRAPHY; ANTIOXIDANT ACTIVITY; VITAMIN-C; METAANALYSIS; SAPONINS; BEANS; SEEDS; QUANTIFICATION;

D O I：

10.1016/j.foodchem.2016.08.113

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

We investigated the metabolite profile of soybean sprouts at 0, 1, 2, 3, and 4 days after germination using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-MS (LC-MS) to understand the relationship between germination and nutritional quality. Data were analyzed by partial least squares-discriminant analysis (PLS-DA), and sprout samples were separated successfully using their PLS-DA scores. Fifty-eight metabolites, including macromolecular derivatives related to energy production, amino acids, myo-inositol metabolites, phytosterols, antioxidants, isoflavones, and soyasaponins, contributed to the separation. Amino acids, myo-inositol metabolites, isoflavone aglycones, B soyasaponins, antioxidants, and phytosterols, associated with health benefits and/or taste quality, increased with germination time while isoflavone glycosides and DDMP soyasaponins decreased. Based on these metabolites, the metabolomic pathway associated with energy production in soybean sprouts is suggested. Our data suggest that sprouting is a useful processing step to improve soybean nutritional quality, and metabolomic analysis is useful in understanding nutritional change during sprouting. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：311 / 319

页数：9

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