Serum Cytokine Profile by ELISA in Patients with Echinococcal Cysts of the Liver: A Stage-Specific Approach to Assess Their Biological Activity

被引：16

作者：

Piccoli, Luca ^{[1
,2
]}

Meroni, Valeria ^{[1
]}

Genco, Francesca ^{[1
]}

Tamarozzi, Francesca ^{[2
]}

Tinelli, Carmine ^{[3
]}

Filice, Carlo ^{[2
]}

Brunetti, Enrico ^{[2
]}

机构：

[1] Univ Pavia, IRCCS San Matteo Hosp Fdn, Dept Infect Dis, Parasitol Lab, I-27100 Pavia, Italy

[2] WHO Collaborating Ctr Clin Management Cyst Echino, I-27100 Pavia, Italy

[3] IRCCS San Matteo Hosp Fdn, Clin Epidemiol & Biometr Unit, I-27100 Pavia, Italy

来源：

CLINICAL & DEVELOPMENTAL IMMUNOLOGY | 2012年

关键词：

BLOOD MONONUCLEAR-CELLS; GRANULOSUS ANTIGEN-B; IMMUNE-RESPONSE; FOLLOW-UP; MECHANISMS;

D O I：

10.1155/2012/483935

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To investigate the usefulness of serum cytokine dosage in the clinical management of cystic echinococcosis (CE), we analyzed serum levels of Th1 and Th2 cytokines in patients with hepatic CE in different cyst stages, CE1-2 (active), CE3a-3b (transitional), and CE4-5 (inactive). Ex vivo assessment of Th1 (IFN-gamma) and Th2 (IL-4, IL-13, and IL-10) cytokines in sera was carried out using ELISA. IL-10 was undetectable in all serum samples of patients and controls, while a few sera contained measurable amounts of IFN-gamma, IL-4, and IL-13. No statistically significant difference was found between the percentages of positive samples for each cytokine and the different groups analyzed (patients/controls, stage, number, location, and size of the cyst, serology, and sex of patients), with the exception of the association of IL-4 and IL-13 with the cyst stage. Overall, this investigation showed many limits of serum cytokine dosage as a marker of biological activity of echinococcal cysts. Because of low sensitivity and lack of specificity of this test, we believe that other ways to evaluate ex vivo biological activity of the cysts should be explored.

引用

页数：5