Structure and anti-tumor activity of a high-molecular-weight polysaccharide from cultured mycelium of Cordyceps gunnii

被引：81
作者：
Zhu, Zhen-yuan [1 ]
Liu, Nian [1 ]
Si, Chuan-ling [2 ]
Liu, Yang [1 ]
Ding, Li-na [1 ]
Jing, Chen [1 ]
Liu, An-jun [1 ]
Zhang, Yong-min [3 ]
机构：
[1] Tianjin Univ Sci & Technol, Coll Food Sci & Biotechnol, Minist Educ, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China
[2] Tianjin Univ Sci & Technol, Coll Mat Sci & Chem Engn, Tianjin Key Lab Pulp & Paper, Tianjin 300457, Peoples R China
[3] Univ Paris 06, Inst Parisien Chim Mol UMR CNRS 7201, F-75005 Paris, France
来源：
CARBOHYDRATE POLYMERS | 2012年 / 88卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Cordyceps gunnii; High-molecular-weight polysaccharide; Characteristic; Antitumor; ANTIOXIDANT ACTIVITY; SINENSIS; PURIFICATION;
D O I：
10.1016/j.carbpol.2012.01.068
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Cordyceps gunnii (berk.) Berk (C. Gunnii) is well known as a Chinese rare caterpillar fungus and has similar pharmacological activity with C. sinensis. In this work, a high-molecular-weight polysaccharide (CPS) was isolated and purified from the mycelia of C. gunnii. The total sugar content of CPS was amounted to 92.84%. The result of HPLC indicated that CPS was a homogeneous polysaccharide. The estimated average molecular weight of CPS was 3.72 x 10(6) Da. The specific rotation of CPS was recorded [alpha](D)(25) = +134.2 degrees. Its characteristic was determined by chemical analysis, gas chromatography, IR spectroscopy and NMR data. The results showed that CPS was mainly composed of glucose, and a small amount of rhamnose, arabinose, xylose, mannose and galactose with a molar ratio of Rha:Ara:Xyl:Man:Glu:Gal = 3.0:2.6:1.0:1.3:106.0:2.8. The main chain of CPS was majorly composed of alpha-(1 -> 4) glucose. The tumor inhibition ratio on K562 cell by CPS was 56.65%. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1072 / 1076
页数：5
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