Granzyme A as a potential biomarker of Mycobacterium tuberculosis infection and disease

被引：13
作者：
Guggino, Giuliana [1 ,2 ]
Orlando, Valentina [1 ,2 ]
Cutrera, Stella [1 ,2 ]
La Manna, Marco P. [1 ,2 ]
Di Liberto, Diana [1 ,2 ]
Vanini, Valentina [3 ]
Petruccioli, Elisa [3 ]
Dieli, Francesco [1 ,2 ]
Goletti, Delia [3 ]
Caccamo, Nadia [1 ,2 ]
机构：
[1] Univ Palermo, Cent Lab Adv Diag & Biomed Res CLADIBIOR, Palermo, Italy
[2] Univ Palermo, Dipartimento Biopatol & Biotecnol Med, Palermo, Italy
[3] Inst Infect Dis L Spallanzani, Rome, Italy
来源：
IMMUNOLOGY LETTERS | 2015年 / 166卷 / 02期
基金：
欧盟第七框架计划;
关键词：
Granzyme A; IFN-gamma; IGRA tests; Tuberculosis; Infection; Disease; GRANULYSIN LEVELS; T-CELLS; DIAGNOSIS; RESPONSES; RELEASE; ASSAYS;
D O I：
10.1016/j.imlet.2015.05.019
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Cytotoxic molecules such as granulysin, perforin and granzymes produced by cytolytic T cells directly contribute to immune defense against tuberculosis (TB). In search for novel TB biomarkers, we have evaluated the levels of granzyme A in plasma obtained from QuantiFERON-TB Gold In tube (QFT-IT) assays from patients with active TB disease and subjects with latent TB infection (LTBI). Granzyme A serum levels in TB patients were significantly lower than values found in LTBI subjects even after subtraction of the unstimulated levels from the antigen-stimulated responses. The receiver operator characteristics (ROC) curve analysis comparing TB patients and LTBI groups, showed that at a cut-off value of granzyme A of <3.425pg/ml, the sensitivity and the specificity of the assay were 29.41% and 94.74%, respectively. Our results suggest that granzyme A could be considered another biomarker of TB, that can be used, other than IFN-gamma, to discriminate between patients with active TB and LTBI subjects in a well characterized cohort of confirmed Mycobacterium tuberculosis-infected individuals. (C) 2015 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.
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收藏
页码：87 / 91
页数：5
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