Identification and Characterization of ABA-Responsive MicroRNAs in Rice

被引:41
|
作者
Tian, Caijuan [1 ,2 ]
Zuo, Zhangli [1 ,2 ]
Qiu, Jin-Long [1 ]
机构
[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
基金
中国国家自然科学基金;
关键词
Rice; Abscisic acid; High throughput sequencing; MicroRNA; Target gene; GENE-EXPRESSION; SIGNAL-TRANSDUCTION; STRESS RESPONSES; ABSCISIC-ACID; PLANT; BIOGENESIS; PROTEIN; ACCUMULATION; INHIBITION; EVOLUTION;
D O I
10.1016/j.jgg.2015.04.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
MicroRNAs (miRNAs) are endogenous non-coding small RNAs that silence genes through mRNA degradation or translational inhibition. The phytohormone abscisic acid (ABA) is essential for plant development and adaptation to abiotic and biotic stresses. In Arabidopsis, miRNAs are implicated in ABA functions. However, ABA-responsive miRNAs have not been systematically studied in rice. Here high throughput sequencing of small RNAs revealed that 107 miRNAs were differentially expressed in the rice ABA deficient mutant, Osaba1. Of these, 13 were confirmed by stem-loop RT-PCR. Among them, miR1425-5P, miR169a, miR169n, miR390-5P, miR397a and miR397b were up-regulated, but miR162b reduced in expression in Osaba1. The targets of these 13 miRNAs were predicted and validated by gene expression profiling. Interestingly, the expression levels of these miRNAs and their targets were regulated by ABA. Cleavage sites were detected on 7 of the miRNA targets by 50-Rapid Amplification of cDNA Ends (50-RACE). Finally, miR162b and its target OsTRE1 were shown to affect rice resistance to drought stress, suggesting that miR162b increases resistance to drought by targeting OsTRE1. Our work provides important information for further characterization and functional analysis of ABA-responsive miRNAs in rice.
引用
收藏
页码:393 / 402
页数:10
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