The expression and role of stromal cell-derived factor-1α-CXCR4 axis in human dental pulp

被引：68
作者：
Jiang, Long [1 ]
Zhu, Ya-Qin [1 ]
Du, Rong [2 ]
Gu, Ying-Xin [2 ]
Xia, Lie [2 ]
Qin, Feng [2 ]
Ritchie, Helena H. [3 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9,Shanghai Res Inst Stomatol, Dept Gen Dent, Shanghai 200011, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9,Shanghai Res Inst Stomatol, Dept Operat Dent & Endodont, Shanghai 200011, Peoples R China
[3] Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA
来源：
JOURNAL OF ENDODONTICS | 2008年 / 34卷 / 08期
关键词：
dental pulp cells; migration; proliferation; stromal cell-derived factor-1 alpha-CXCR4 axis; tissue repair;
D O I：
10.1016/j.joen.2008.05.015
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Recent reports have suggested that the stromal cell-derived factor (SDF)-1 alpha-CXCR4 axis has a direct effect on stem and progenitor cell recruitment in muscle and neural tissue repair after injury. No information is available about SDF-1 alpha or CXC chemokine receptor 4 (CXCR4) in dental tissues. The aim of this study was to assess the expression of SDF-1 alpha and its receptor, CXCR4, in healthy or inflamed human dental pulp and to evaluate the effects of SDF-1 alpha on dental pulp cells (DPCs) in both proliferation and migration in vitro. Immunohistochemical staining and reverse-transcription polymerase chain reaction detected weak expression of SDF-1 alpha and CXCR4 in healthy dental pulp and strong expression of SDF-1 alpha and CXCR4 in inflamed dental pulp. A methylthiazol tetrazolium assay showed that SDF-1 alpha could not promote DPCs proliferation. A transmigration assay, however, indicated that SDF-1 alpha enhanced DPCs migration, which could be abolished by anti-CXCR4 antibodies. Taken together, these results imply that the SDF-1 alpha-CXCR4 axis may play a role in the recruitment of CXCR4-positive DPCs toward the damaged sites.
引用
收藏
页码：939 / 944
页数：6
相关论文
共 22 条
[1]   Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1 [J].
Ceradini, DJ ;
Kulkarni, AR ;
Callaghan, MJ ;
Tepper, OM ;
Bastidas, N ;
Kleinman, ME ;
Capla, JM ;
Galiano, RD ;
Levine, JP ;
Gurtner, GC .
NATURE MEDICINE, 2004, 10 (08) :858-864
[2]   Investigation of CXCR4 in squamous cell carcinoma of the tongue [J].
Delilbasi, CB ;
Okura, M ;
Iida, S ;
Kogo, M .
ORAL ONCOLOGY, 2004, 40 (02) :154-157
[3]   Cells and extracellular matrices of dentin and pulp: A biological basis for repair and tissue engineering [J].
Goldberg, M ;
Smith, AJ .
CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE, 2004, 15 (01) :13-27
[4]   Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J].
Gronthos, S ;
Mankani, M ;
Brahim, J ;
Robey, PG ;
Shi, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13625-13630
[5]   Evaluation of the cytocompatibility of three endodontic materials [J].
Guigand, M ;
Pellen-Mussi, P ;
Le Goff, A ;
Vulcain, JM ;
Bonnaure-Mallet, M .
JOURNAL OF ENDODONTICS, 1999, 25 (06) :419-423
[6]   CXCL12 and CXCR4 expression by human gingival fibroblasts in periodontal disease [J].
Hosokawa, Y ;
Hosokawa, I ;
Ozaki, K ;
Nakae, H ;
Murakami, K ;
Miyake, Y ;
Matsuo, T .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2005, 141 (03) :467-474
[7]   Directed migration of neural stem cells to sites of CNS injury by the stromal cell-derived factor 1α/CXC chemokine receptor 4 pathway [J].
Imitola, J ;
Raddassi, K ;
Park, KI ;
Mueller, FJ ;
Nieto, M ;
Teng, YD ;
Frenkel, D ;
Li, JX ;
Sidman, RL ;
Walsh, CA ;
Snyder, EY ;
Khoury, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (52) :18117-18122
[8]   Regulation of SDF-1 (CXCL12) production by osteoblasts; a possible mechanism for stem cell homing [J].
Jung, Y ;
Wang, J ;
Schneider, A ;
Sun, YX ;
Koh-Paige, AJ ;
Osman, NI ;
McCauley, LK ;
Taichman, RS .
BONE, 2006, 38 (04) :497-508
[9]   Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: Pivotal role of the SDF-1-CXCR4 axis [J].
Kucia, M ;
Reca, R ;
Miekus, K ;
Wanzeck, J ;
Wojakowski, W ;
Janowska-Wieczorek, A ;
Ratajczak, J ;
Ratajczak, MZ .
STEM CELLS, 2005, 23 (07) :879-894
[10]   Chemokines - Chemotactic cytokines that mediate inflammation [J].
Luster, AD .
NEW ENGLAND JOURNAL OF MEDICINE, 1998, 338 (07) :436-445
123