Thioamide quenching of intrinsic protein fluorescence

被引：58

作者：

Goldberg, Jacob M. ^{[1
]}

Wissner, Rebecca F. ^{[1
]}

Klein, Alyssa M. ^{[1
]}

Petersson, E. James ^{[1
]}

机构：

[1] Univ Penn, Dept Chem, Philadelphia, PA 19104 USA

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 10期

基金：

美国国家科学基金会;

关键词：

RESONANCE ENERGY-TRANSFER; TRYPTOPHAN FLUORESCENCE; BINDING; CALMODULIN; ELECTRON; CALCIUM; TYROSINE; PEPTIDE; PROBES;

D O I：

10.1039/c1cc14708k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Thioamides quench tryptophan and tyrosine fluorescence in a distance-dependent manner and thus can be used to monitor the binding of thioamide-containing peptides to proteins. Since thioamide analogs of the natural amino acids can be synthetically incorporated into peptides, they can function as minimally-perturbing probes of protein/peptide interactions.

引用

收藏

页码：1550 / 1552

页数：3

相关论文

共 32 条

[1]

BEECHEM JM, 1985, ANNU REV BIOCHEM, V54, P43, DOI 10.1146/annurev.biochem.54.1.43

[2] THE RELATIVE EASE OF REMOVING A PROTON, A HYDROGEN-ATOM, OR AN ELECTRON FROM CARBOXAMIDES VERSUS THIOCARBOXAMIDES [J].

BORDWELL, FG ;

ALGRIM, DJ ;

HARRELSON, JA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (17) :5903-5904

[3] Pitfalls and limitations in the practical use of Forster's theory of resonance energy transfer [J].

Braslavsky, Silvia E. ;

Fron, Eduard ;

Rodriguez, Hernan B. ;

Roman, Enrique San ;

Scholes, Gregory D. ;

Schweitzer, Gerd ;

Valeur, Bernard ;

Wirz, Jakob .

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2008, 7 (12) :1444-1448

[4] Fluorescence spectroscopy as a tool to investigate protein interactions [J].

Brown, MP ;

Royer, C .

CURRENT OPINION IN BIOTECHNOLOGY, 1997, 8 (01) :45-49

[5] FLUORESCENCE ENERGY-TRANSFER ANALYSIS OF CALMODULIN.PEPTIDE COMPLEXES [J].

CHAPMAN, ER ;

ALEXANDER, K ;

VORHERR, T ;

CARAFOLI, E ;

STORM, DR .

BIOCHEMISTRY, 1992, 31 (51) :12819-12825

[6] Toward understanding tryptophan fluorescence in proteins [J].

Chen, Y ;

Barkley, MD .

BIOCHEMISTRY, 1998, 37 (28) :9976-9982

[7] The peptide bond quenches indole fluorescence [J].

Chen, Y ;

Liu, B ;

Yu, HT ;

Barkley, MD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (39) :9271-9278

[8] Structure of calmodulin complexed with an olfactory CNG channel fragment and role of the central linker: Residual dipolar couplings to evaluate calmodulin binding modes outside the kinase family [J].

Contessa, GM ;

Orsale, M ;

Melino, S ;

Torre, V ;

Paci, M ;

Desideri, A ;

Cicero, DO .

JOURNAL OF BIOMOLECULAR NMR, 2005, 31 (03) :185-199

[9] Probing polyproline structure and dynamics by photoinduced electron transfer provides evidence for deviations from a regular polyproline type II helix [J].

Doose, Soeren ;

Neuweiler, Hannes ;

Barsch, Hannes ;

Sauer, Markus .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (44) :17400-17405

[10]

EFTINK MR, 1991, METHOD BIOCHEM ANAL, V35, P127

← 1 2 3 4 →