A transient assay for rapid functional analysis of transcription factors in arabidopsis

被引：13

作者：

Sessa, G

Borello, U

Morelli, G

Ruberti, I

机构：

[1] Univ Rome La Sapienza, Dipartimento Genet & Biol Mol, Ctr Studio Acidi Nucl, I-00185 Rome, Italy

[2] Ist Nazl Nutr, Unit Nutr Sperimentale, I-00178 Rome, Italy

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1998年 / 16卷 / 02期

关键词：

Arabidopsis thaliana; ATHB-1; microprojectile-mediated gene transfer; transcription factors; transient assay;

D O I：

10.1023/A:1007442607645

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have modified the biolistic process to establish a transient assay for rapid analysis of transcription factors in arabidopsis. ATHB-1, an arabidopsis HD-Zip protein, was used as a transacting factor in particle bombardment experiments with arabidopsis leaves, and was found to transactivate a reporter gene in a binding site-specific manner. The experiments show the feasibility of using microprojectile-mediated gene transfer for rapid functional analysis of transcription factors in arabidopsis.

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收藏

页码：191 / 197

页数：7

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