Polysaccharides derived from Lycium barbarum suppress IGF-1-induced angiogenesis via PI3K/HIF-1α/VEGF signalling pathways in MCF-7 cells

被引：34

作者：

Huang, Xu ^{[2
]}

Zhang, Qing-Yuan ^{[1
]}

Jiang, Qiu-Ying ^{[3
]}

Kang, Xin-Mei ^{[1
]}

Zhao, Ling ^{[1
]}

机构：

[1] Harbin Med Coll, Affiliated Hosp 3, Dept Med Oncol, Harbin 150040, Peoples R China

[2] Harbin Med Coll, Affiliated Hosp 3, Dept Radiat Oncol, Harbin 150040, Peoples R China

[3] Harbin Med Coll, Affiliated Hosp 2, Dept Med Oncol, Harbin 150040, Peoples R China

来源：

FOOD CHEMISTRY | 2012年 / 131卷 / 04期

关键词：

LBPs; Breast cancer; IGF-1; PI3K; HIF-1; alpha; VEGF; Angiogenesis; HUMAN BREAST-CANCER; GROWTH-FACTOR-I; TUMOR ANGIOGENESIS; PHYTOCHEMICALS; HYPOXIA; INSULIN; APOPTOSIS; INHIBIT; EXTRACT; CHEMOPREVENTION;

D O I：

10.1016/j.foodchem.2011.10.039

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Lycium barbarum (LB) is a berry-type fruit. Lycium barbarum polysaccharides (LBPs), derived from LB, has anti-tumour properties and exhibits potent anti-angiogenic effects. The aim of this study was to elucidate possible signal transduction pathways functioning to mediate the breast cancer-suppressive effects of LBPs. Using MCF-7 cells, we determined that a 90 h treatment with 0.50 mg/ml of LBPs resulted in significant inhibition of cell proliferation. Using this same system, we observed that LBPs could also affect insulin-like growth factor (IGF)-1 protein accumulation, suppress phosphatidylinositol 3-kinase (PI3K) activity and phosphorylated-PI3K (p-PI3K) protein levels, inhibit hypoxia-inducible factor-1 (HIF-1 alpha) protein accumulation without altering HIF-1 alpha mRNA levels, and suppress VEGF mRNA expression and protein production. Our results demonstrate that LBPs may inhibit tumour cell growth by suppressing IGF-1-induced angiogenesis via PI3K/HIF-1 alpha/VEGF signalling pathways. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

引用

页码：1479 / 1484

页数：6

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