Immunochemical studies on human, bovine and pig brain regulatory subunits of cAMP-dependent protein kinase type II

被引：0
|
作者：
Sveshnikova, EV
Alexandrova, NA
Grozdova, ID
Sveshnikov, PG
Severin, ES
机构：
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1996年 / 39卷 / 05期
关键词：
c-AMP-dependent protein kinase; regulatory subunit type II; monoclonal antibodies;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Regulatory subunits type II (RII) purified from human, pig and bovine brains were compared using 11 monoclonal antibodies (MoAb) against bovine brain RII. Bovine RII has al least 5 antigenic sites located in the N-terminal 1-110 residues. Immunochemical difference detected between human and animal RII was more pronounced than between pin and bovine RII. Certain MoAb influenced R-cAMP binding and holoenzyme formation. RII of the three species responded to MoAb attachment in a similar fashion. The results suggest that anchoring of neural protein kinase via the N-terminal part of RII may influence the enzyme activity.
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页码：1063 / 1070
页数：8
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