MCPIP1 attenuates the innate immune response to influenza A virus by suppressing RIG-I expression in lung epithelial cells

被引:19
作者
Sun, Xiaoning [1 ]
Feng, Wenjing [1 ]
Guo, Yidi [1 ]
Wang, Qi [1 ]
Dong, Chunyan [1 ]
Zhang, Maolin [1 ]
Guan, Zhenhong [1 ]
Duan, Ming [1 ]
机构
[1] Jilin Univ, Inst Zoonosis, Coll Vet Med, Key Lab Zoonoses Res,Minist Educ, Changchun, Jilin, Peoples R China
关键词
influenza virus; MCPIP1; NS1; RIG-I; MESSENGER-RNA; NS1; PROTEIN; ANTIVIRAL RESPONSE; GENE-EXPRESSION; RECOGNITION; INTERFERON; INDUCTION; INFECTION; REPLICATION; DEGRADATION;
D O I
10.1002/jmv.24944
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The pattern recognition receptor retinoic acid-inducible gene I (RIG-I) reportedly plays a key role in sensing influenza A virus (IAV) infection and activating type I interferon (IFN) response. MCP-1-induced protein 1 (MCPIP1) can directly degrade cytokine mRNAs, such as IL-6, IL-12, IL-1, and IL-2, by functioning as an RNase. Here, we initially observed that MCPIP1 exhibited virus supportive functions later in the course of IAV infection in A549 cells, and negatively regulated IAV-induced RIG-I-dependent innate antiviral response. Exogenous overexpression of MCPIP1 suppressed the expression of RIG-I, whereas shRNA-mediated inhibition of endogenous MCPIP1 enhanced RIG-I expression. The results of experiments with actinomycin D and luciferase assay demonstrated that MCPIP1 reduced RIG-I expression through destabilizing its mRNA. Various mutants of functional domains of MCPIP1 further confirmed that the inhibitory effect of MCPIP1 on RIG-I expression required RNase activity but not deubiquitinase activity. Finally, the overexpression of several IAV proteins, which have the ability to inhibit the host IFN response at different levels, induced MCPIP1 expression, especially non-structural protein 1 (NS1). Conclusively, these data demonstrate the MCPIP1 contributes to attenuate IAV-induced host antiviral response by suppressing RIG-I expression.
引用
收藏
页码:204 / 211
页数:8
相关论文
共 34 条
  • [1] MCPIP-1, Alias Regnase-1, Controls Epithelial Inflammation by Posttranscriptional Regulation of IL-8 Production
    Dobosz, Ewelina
    Wilamowski, Mateusz
    Lech, Maciej
    Bugara, Beata
    Jura, Jolanta
    Potempa, Jan
    Koziel, Joanna
    [J]. JOURNAL OF INNATE IMMUNITY, 2016, 8 (06) : 564 - 578
  • [2] Modulation of influenza A virus replication by microRNA-9 through targeting MCPIP1
    Dong, Chunyan
    Sun, Xiaoning
    Guan, Zhenhong
    Zhang, Maolin
    Duan, Ming
    [J]. JOURNAL OF MEDICAL VIROLOGY, 2017, 89 (01) : 41 - 48
  • [3] Influenza A viruses suppress cyclooxygenase-2 expression by affecting its mRNA stability
    Dudek, Sabine Eva
    Nitzsche, Katja
    Ludwig, Stephan
    Ehrhardt, Christina
    [J]. SCIENTIFIC REPORTS, 2016, 6
  • [4] Influenza A Virus NS1 Targets the Ubiquitin Ligase TRIM25 to Evade Recognition by the Host Viral RNA Sensor RIG-I
    Gack, Michaela Ulrike
    Albrecht, Randy Allen
    Urano, Tomohiko
    Inn, Kyung-Soo
    Huang, I-Chueh
    Carnero, Elena
    Farzan, Michael
    Inoue, Satoshi
    Jung, Jae Ung
    Garcia-Sastre, Adolfo
    [J]. CELL HOST & MICROBE, 2009, 5 (05) : 439 - 449
  • [5] Innate immunity to influenza virus infection
    Iwasaki, Akiko
    Pillai, Padmini S.
    [J]. NATURE REVIEWS IMMUNOLOGY, 2014, 14 (05) : 315 - 328
  • [6] RIG-I Signaling Is Critical for Efficient Polyfunctional T Cell Responses during Influenza Virus Infection
    Kandasamy, Matheswaran
    Suryawanshi, Amol
    Tundup, Smanla
    Perez, Jasmine T.
    Schmolke, Mirco
    Manicassamy, Santhakumar
    Manicassamy, Balaji
    [J]. PLOS PATHOGENS, 2016, 12 (07)
  • [7] Cell type-specific involvement of RIG-I in antiviral response
    Kato, H
    Sato, S
    Yoneyama, M
    Yamamoto, M
    Uematsu, S
    Matsui, K
    Tsujimura, T
    Takeda, K
    Fujita, T
    Takeuchi, O
    Akira, S
    [J]. IMMUNITY, 2005, 23 (01) : 19 - 28
  • [8] Klimov Alexander, 2012, Methods Mol Biol, V865, P25, DOI 10.1007/978-1-61779-621-0_3
  • [9] NF-κB Signaling Differentially Regulates Influenza Virus RNA Synthesis
    Kumar, Naveen
    Xin, Zhong-tao
    Liang, Yuhong
    Ly, Hinh
    Liang, Yuying
    [J]. JOURNAL OF VIROLOGY, 2008, 82 (20) : 9880 - 9889
  • [10] Cutting edge: Influenza A virus activates TLR3-dependent inflammatory and RIG-1-dependent antiviral responses in human lung epithelial cells
    Le Goffic, Ronan
    Pothlichet, Julien
    Vitour, Damien
    Fujita, Takashi
    Meurs, Eliane
    Chignard, Michel
    Si-Tahar, Mustapha
    [J]. JOURNAL OF IMMUNOLOGY, 2007, 178 (06) : 3368 - 3372