Taxonomic and genetic status of lancelet in Weihai coastal waters based on mitochondrial DNA sequence

被引:5
作者
Zhao Qi [1 ]
Zhu Qian [1 ,2 ]
机构
[1] Shandong Univ Weihai, Ocean Coll, Weihai 264209, Peoples R China
[2] State Ocean Adm, Inst Oceanog 3, Xiamen 361005, Peoples R China
关键词
lancelet; Cyt b; CO I; taxonomic status; genetic status; CYTOCHROME-B; PHYLOGENETIC-RELATIONSHIPS; INTEGRATED SOFTWARE; POPULATION; DIVERSITY; EVOLUTION; GENOME; DIFFERENTIATION; PHYLOGEOGRAPHY; BRANCHIOSTOMA;
D O I
10.1007/s00343-011-0131-6
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Lancelets (subphylum Cephalochordata) are a transitional species between invertebrates and vertebrates. They are currently listed in the Second Order of Protected Animals in China. Lancelets were first documented in the waters around the city of Weihai (Shandong, China) in 2002. However, little is known about the phylogeny of this population. We analyzed the sequences of cytochrome b (Cyt b) and cytochrome oxidase c subunit I (CO I) genes from samples collected from coastal waters in the cities of Weihai and Qingdao (similar to 150 km to the south). We analyzed 176 sequences, of which 150 were novel sequences and 26 were obtained from GenBank. Our results suggest that (1) lancelets in the two cities belong to the species Branchiostoma japonicus and have a high level of genetic diversity; (2) there is a high level of gene flow and low level of genetic differentiation between lancelets from the two cities; (3) demographic expansion occurred an estimated 1.1 million years (Ma) ago (mid Pleistocene) for lancelets in Weihai-Qingdao; and (4) the divergence between B. belcheri and B. japonicus was estimated at between 37.75 Ma (early Oligocene)-46.5 Ma (late Eocene).
引用
收藏
页码:623 / 632
页数:10
相关论文
共 47 条
[31]   MtDNA genetic diversity and population history of a dwindling raptorial bird, the red kite (Milvus milvus) [J].
Roques, S ;
Negro, JJ .
BIOLOGICAL CONSERVATION, 2005, 126 (01) :41-50
[32]   DnaSP, DNA polymorphism analyses by the coalescent and other methods [J].
Rozas, J ;
Sánchez-DelBarrio, JC ;
Messeguer, X ;
Rozas, R .
BIOINFORMATICS, 2003, 19 (18) :2496-2497
[33]  
Sun Yu-miao, 2008, Marine Sciences (Beijing), V32, P18
[34]  
TAJIMA F, 1989, GENETICS, V123, P585
[35]  
TAKAHATA N, 1985, GENETICS, V109, P441
[36]   The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools [J].
Thompson, JD ;
Gibson, TJ ;
Plewniak, F ;
Jeanmougin, F ;
Higgins, DG .
NUCLEIC ACIDS RESEARCH, 1997, 25 (24) :4876-4882
[37]  
WANG LJ, 1996, QUATERNARY SCI, V4, P300
[38]  
Wang Yi-quan, 2005, Zoological Research, V26, P666
[39]  
Wang Yi-Quan, 2004, Acta Zoologica Sinica, V50, P202
[40]  
WEI Q, 2004, MOL BIOL LAB PROCEDU, P66