Silver and Copper Nanoparticles Induce Oxidative Stress in Bacteria and Mammalian Cells

被引：58

作者：

Ameh, Thelma ^{[1
]}

Gibb, Matthew ^{[2
]}

Stevens, Dinny ^{[1
]}

Pradhan, Sahar H. ^{[1
]}

Braswell, Evan ^{[3
]}

Sayes, Christie M. ^{[1
,2
]}

机构：

[1] Baylor Univ, Dept Environm Sci, Waco, TX 76798 USA

[2] Baylor Univ, Inst Biomed Studies, Waco, TX 76798 USA

[3] Anim & Plant Hlth Inspect Serv, Mission Lab, USDA, Plant Protect & Quarantine Sci & Technol, Edinburg, TX 78541 USA

来源：

NANOMATERIALS | 2022年 / 12卷 / 14期

关键词：

cetyltrimethylammonium bromide; polyvinyl pyrrolidone; reactive oxygen species; metal ion; E; coli; BEAS-2B; ESCHERICHIA-COLI; ANTIBACTERIAL ACTIVITY; ANTIMICROBIAL ACTIVITY; OXIDE NANOPARTICLES; LIPID-PEROXIDATION; ZNO NANOPARTICLES; BEAS-2B CELLS; DNA-DAMAGE; IN-VITRO; MECHANISMS;

D O I：

10.3390/nano12142402

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Silver and copper nanoparticles (AgNPs and CuNPs) coated with stabilizing moieties induce oxidative stress in both bacteria and mammalian cells. Effective antibacterial agents that can overcome existing mechanisms of antibacterial resistance will greatly improve biomedical interventions. In this study, we analyzed the effect of nanoparticle-induced stress. Escherichia coli and normal human bronchial epithelial (BEAS-2B) cells were selected for this study. The nanoparticle constructs tested showed low toxicity to mammalian cells except for the polyvinylpyrrolidone-surface-stabilized copper nanoparticles. In fact, both types of copper nanoparticles used in this study induced higher levels of reactive oxygen species than the surface-stabilized silver nanoparticles. In contrast to mammalian cells, the surface-stabilized silver and copper nanoparticles showed varying levels of toxicity to bacteria cells. These data are expected to aid in bridging the knowledge gap in differential toxicities of silver and copper nanoparticles against bacteria and mammalian cells and will also improve infection interventions.

引用

页数：13

共 59 条

[1] In vitro evaluation of organic extractable matter from ambient PM2.5 using human bronchial epithelial BEAS-2B cells: Cytotoxicity, oxidative stress, pro-inflammatory response, genotoxicity, and cell cycle deregulation [J].