Activation of Rac1 by a Crk SH3-binding protein, DOCK180

被引：368

作者：

Kiyokawa, E

Hashimoto, Y

Kobayashi, S

Sugimura, H

Kurata, T

Matsuda, M ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Pathol, Shinjuku Ku, Tokyo 1628640, Japan

[2] Hamamatsu Univ Sch Med, Dept Pathol 1, Hamamatsu, Shizuoka 4313192, Japan

[3] Int Med Ctr Japan, Inst Res, Dept Pathol, Shinjuku Ku, Tokyo 1628655, Japan

来源：

GENES & DEVELOPMENT | 1998年 / 12卷 / 21期

关键词：

DOCK180; Crk; Rac; mbc; ced-5; guanine nucleotide exchange factor;

D O I：

10.1101/gad.12.21.3331

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

DOCK180 is involved in integrin signaling through CrkII-p130(Cas) complexes. We have studied the involvement of DOCK180 in Rad signaling cascades. DOCK180 activated JNK in a manner dependent on Rad, Cdc42Hs, and SEK, and overexpression of DOCK180 increased the amount of GTP-bound Rad in 293T cells. Coexpression of CrkII and p130(Cas) enhanced this DOCK180-dependent activation of Rad. Furthermore, we observed direct binding of DOCK180 to Rad, but not to RhoA or Cdc42Hs. Dominant-negative Rad suppressed DOCK180-induced membrane spreading. These results strongly suggest that DOCK180 is a novel activator of Rad and involved in integrin signaling.

引用

页码：3331 / 3336

页数：6

共 35 条

[1] The Dbl family of oncogenes [J].