Biological activity and safety profile of the essential oil from fruits of Heracleum mantegazzianum Sommier & Levier (Apiaceae)

被引：20

作者：

Skalicka-Wozniak, Krystyna ^{[1
]}

Grzegorczyk, Agnieszka ^{[2
]}

Swiatek, Lukasz ^{[3
]}

Walasek, Magdalena ^{[1
]}

Widelski, Jaroslaw ^{[1
]}

Rajtar, Barbara ^{[3
]}

Polz-Dacewicz, Malgorzata ^{[3
]}

Maim, Anna ^{[2
]}

Elansary, Hosam O. ^{[4
]}

机构：

[1] Med Univ Lublin, Med Plant Unit, Dept Pharmacognosy, 1 Chodzki Str, PL-20093 Lublin, Poland

[2] Med Univ Lublin, Lab Microbiol Diagnost, Chair & Dept Pharmaceut Microbiol, Lublin, Poland

[3] Med Univ Lublin, Dept Virol, Lublin, Poland

[4] Alexandria Univ, Fac Agr El Shatby, Dept Floriculture Ornamental Hort & Garden Design, Alexandria, Egypt

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2017年 / 109卷

关键词：

Heracleum mantegazzianum; Giant hogweed; Antimicrobial activity; Cytotoxicity; Umbelliferae; TERNATUM VELEN. BRUMMITT; CHEMICAL-COMPOSITION; OCTYL ACETATE; ANTIOXIDANT; IDENTIFICATION; COMPONENTS; HOGWEED; FUNGI;

D O I：

10.1016/j.fct.2017.05.033

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A composition of essential oils obtained from Heracleum mantegazzianum (Apiaceae) was examined using a GC-MS method. n-Octyl acetate (19.92%), n-hexyl-2-methylbutanoate (10.84%), n-octanol (10.13%), n-octyl butanoate (8.88%), n-octyl-2-methylbutanoate (8.01%), n-hexyl acetate (7.11%), n-octyl isobutanoate (5.5%) and n-hexyl isobutanoate (5.43%) were the main compounds. The high-performance counter-current chromatography was applied for purification of aliphatic alcohols and esters. A mixture of n-hexane, acetonitrile and tetr-butyl methyl ether (1:1:0.1, v/v) allowed to obtain n-octanol, n-octyl acetate, n-hexyl-2- methylbutanoate, n-octyl isobutanoate and n-octy1-2-methylbutanoate, with the purity range of 94-99%, in one single 74 min run. The antimicrobial activity was also determined against plant and foodborne pathogens. While n-octanol shares responsibility for the antibacterial activity of the essential oil, n-octyl acetate determines its antifungal action. The cytotoxic activity assessed on two normal kidney fibroblast cell lines: Vero (animal) and HEK-293 (human embryonic), and two human cancer cell lines: FaDu (squamous cell carcinoma of the pharynx) and SCC25 (squamous cell carcinoma of the tongue), showed a moderate cytotoxicity with CC50 values ranging from 262.3 to 567.8 mu g/mL. Results indicate that normal cell lines were more sensitive to the tested essential oil than cancer cell lines. The antioxidant activity of oil and pure compounds was not significant. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：820 / 826

页数：7