Ganoderma lucidum polysaccharide enhances radiosensitivity of hepatocellular carcinoma cell line HepG2 through Akt signaling pathway

被引:29
|
作者
Yu, Yang [1 ]
Qian, Liqi [1 ]
Du, Nan [2 ]
Liu, Yuxiao [3 ]
Zhao, Xiao [4 ]
Zhang, Xin [5 ]
机构
[1] Gen Hosp Peoples Liberat Army China, Affiliated Hosp 1, Dept Tradit Chinese Med, 51 Fucheng Rd, Beijing 100048, Peoples R China
[2] Gen Hosp Peoples Liberat Army China, Affiliated Hosp 1, Dept Oncol, 51 Fucheng Rd, Beijing 100048, Peoples R China
[3] Gen Hosp Peoples Liberat Army China, Affiliated Hosp 1, Dept Neurosurg, Beijing 100048, Peoples R China
[4] Gen Hosp Peoples Liberat Army China, Affiliated Hosp 1, Dept Med Adm, Beijing 100048, Peoples R China
[5] Gen Hosp Peoples Liberat Army China, Affiliated Hosp 1, Dept Pharm, Beijing 100048, Peoples R China
关键词
hepatocellular carcinoma; Ganoderma lucidum polysaccharide; radiation; DNA damage; TRADITIONAL CHINESE HERBS; DNA-DAMAGE RESPONSE; OXIDATIVE STRESS; UP-REGULATION; CANCER; APOPTOSIS; INHIBITION; RESISTANCE; ANTITUMOR; GROWTH;
D O I
10.3892/etm.2017.5340
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Ganoderma lucidum polysaccharide (GLP) is a well-known traditional Chinese medicine, known for its anti-cancer and immunomodulatory properties. The present study aims to investigate whether GLP has a therapeutic effect on hepatocellular carcinoma (HCC) cells exposed to radiation. Immunofluorescence was used to detect the nuclei, the protein expression was measured by western blot analysis and flow cytometry was used to detect the rate of cell apoptosis. GLP treatment was demonstrated to enhance radiation-induced growth inhibition and apoptotic death of HCC cells. At a molecular level, GLP suppressed the activities of DNA repair-associated proteins including ataxia-telangiectasia mutated (ATM) and DNA dependent-protein kinase (DNA-PK) in liver cancer cells under radiation conditions. Furthermore, the addition of an Akt inhibitor elevated the activities of DNA-PK and ATM and attenuated the GLP-induced HepG2 cell injury under the radiation condition. In conclusion, the present study demonstrates that GLP enhances the radiosensitivity of HCC cells via the regulation of Akt signaling pathways, implying a potential therapeutic effect of GLP as a radiation sensitizer in HCC treatment.
引用
收藏
页码:5903 / 5907
页数:5
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