A reagentless amperometric hydrogen peroxide biosensor based on covalently binding horseradish peroxidase and thionine using a thiol-modified gold electrode

被引：51
|
作者：
Ruan, CM [1 ]
Yang, R [1 ]
Chen, XH [1 ]
Deng, JQ [1 ]
机构：
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
来源：
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1998年 / 455卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
self-assembled monolayer; horseradish peroxidase; thionine; hydrogen peroxide;
D O I：
10.1016/S0022-0728(98)00153-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new approach to construct a reagentless hydrogen peroxide biosensor is described. Horseradish peroxidase and thionine are covalently bound to a cysteamine-assembled gold electrode using glutaraldehyde as a bifunctional reagent. Thionine immobilised in this way can shuttle electrons between the electrode and the redox activity center of the enzyme. The sensor was highly sensitive to hydrogen peroxide with a detection limit of 8.0 x 10(-7) mol l(-1) and a response time of less than 4 s. The effects of the applied potential and the pH values of the buffer solution on the response of the sensor were investigated for optimum analytical performance. (C) 1998 Elsevier Science S.A. All rights reserved.
引用
收藏
页码：121 / 125
页数：5
相关论文
共 50 条
  • [31] Amperometric hydrogen peroxide biosensor based on a glassy carbon electrode modified with polythionine and gold nanoparticles
    Wang, Qingxia
    Zhang, Haili
    Wu, Yiwei
    Yu, Aimin
    MICROCHIMICA ACTA, 2012, 176 (3-4) : 279 - 285
  • [32] Electrochemical study of Horseradish peroxidase using the nanosilver-modified graphite electrode and its application to hydrogen peroxide biosensor
    Rezaei-Zarchi, Saeed
    Saboury, Ali Akbar
    Javed, Aisha
    JOURNAL OF NEW MATERIALS FOR ELECTROCHEMICAL SYSTEMS, 2008, 11 (03) : 199 - 203
  • [33] A Novel Hydrogen Peroxide Biosensor Based on Adsorption of Horseradish Peroxidase onto a Nanobiomaterial Composite Modified Glassy Carbon Electrode
    Moyo, Mambo
    Okonkwo, Jonathan O.
    Agyei, Nana M.
    ELECTROANALYSIS, 2013, 25 (08) : 1946 - 1954
  • [34] Reagentless chemiluminescence biosensor for determination of hydrogen peroxide based on the immobilization of horseradish peroxidase on biocompatible chitosan membrane
    Zhou, GJ
    Wang, G
    Xu, JJ
    Chen, HY
    SENSORS AND ACTUATORS B-CHEMICAL, 2002, 81 (2-3) : 334 - 339
  • [35] A Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase/Poly(L-leucine)/Polydopamine Modified Glassy Carbon Electrode
    Zheng, Xinyu
    Guo, Yuheng
    Zheng, Jinshuai
    Ma, Cong
    Zhou, Xuechou
    Lin, Jiandi
    Lin, Ruiyu
    RUSSIAN JOURNAL OF ELECTROCHEMISTRY, 2017, 53 (05) : 443 - 451
  • [36] Amperometric Biosensor for Hydrogen Peroxide Based on Electrodeposited Sub-micrometer Gold Modified Glassy Carbon Electrode
    王树青
    陈峻
    林祥钦
    Chinese Journal of Chemistry, 2004, (04) : 360 - 364
  • [37] Amperometric biosensor for hydrogen peroxide based on electrodeposited sub-micrometer gold modified glassy carbon electrode
    Wang, SQ
    Chen, J
    Lin, XQ
    CHINESE JOURNAL OF CHEMISTRY, 2004, 22 (04) : 360 - 364
  • [38] A hydrogen peroxide biosensor based on horseradish peroxidase/poly(L-leucine)/polydopamine modified glassy carbon electrode
    Xinyu Zheng
    Yuheng Guo
    Jinshuai Zheng
    Cong Ma
    Xuechou Zhou
    Jiandi Lin
    Ruiyu Lin
    Russian Journal of Electrochemistry, 2017, 53 : 443 - 451
  • [39] Hydrogen peroxide biosensor based on immobilizing enzyme by gold nanoparticles and thionine
    Chen Xian-Guang
    Qian Ying
    Zhang Su-Juan
    Zou Xiao-Yong
    ACTA CHIMICA SINICA, 2007, 65 (04) : 337 - 343
  • [40] Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template
    Yao, Hui
    Liu, Huan
    Sun, Mei-jiao
    Gong, Lei
    MICROCHIMICA ACTA, 2012, 177 (1-2) : 31 - 37
12345