In vitro Cell Proliferation Assay of Demineralized Dentin Material Membrane in Osteoblastic MC3T3-E1 Cells

被引:3
作者
Soesilawati, Pratiwi [1 ,2 ,3 ,4 ]
Rizqiawan, Andra [4 ,5 ]
Roestamadji, Retno Indrawati [1 ,3 ]
Arrosyad, Ahmad Rizal [6 ]
Firdauzy, Muhammad Alwino Bayu [3 ,6 ]
Abu Kasim, Noor Hayaty [7 ,8 ]
机构
[1] Univ Airlangga, Fac Dent Med, Dept Oral Biol, Jl Mayjend Prof Dr Moestopo 47, Surabaya 60132, Indonesia
[2] Univ Airlangga, Fac Med, Dr Soetomo Gen Acad Hosp, Cell & Tissue Bank Regenerat Med, Surabaya, Indonesia
[3] Univ Airlangga, Postgrad Sch, Immunol Program, Surabaya, Indonesia
[4] Univ Airlangga, Dent Hosp, Surabaya, Indonesia
[5] Univ Airlangga, Fac Dent Med, Dept Oral & Maxillofacial Surg, Surabaya, Indonesia
[6] Univ Airlangga, Fac Dent Med, Dent Profess Program, Surabaya, Indonesia
[7] Univ Kebangsaan Malaysia, Fac Dent, Kuala Lumpur, Malaysia
[8] Univ Airlangga, Fac Dent Med, Surabaya, Indonesia
来源
CLINICAL COSMETIC AND INVESTIGATIONAL DENTISTRY | 2021年 / 13卷
关键词
cells; biomedical and dental materials; oral surgical procedures; materials testing; wound; injuries; cytotoxicity test; pre-prosthetic; BONE; VIABILITY; BOVINE;
D O I
10.2147/CCIDE.S313184
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Aim: Demineralized dentin material membrane (DDMM) is a novel bioresorbable guided bone regeneration (GBR) which is derived from the demineralization process of bovine dentin. This material/process could be an alternative to resolve musculoskeletal dysfunction that harms the quality of human life. Purpose: To evaluate the cytotoxic effect of DDMM as GBR membrane on MC3T3-E1 osteoblast cell line. Methods: Cytotoxic effect was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Osteoblast MC3T3-E1 cell culture was used as a parameter of cell viability after reacting with GBR materials. The absorbance values were examined at each treatment to determine the percentage of cell viability. There were four groups created in the present study: two treatment groups and two control groups. The treatment groups consisted of a DDMM group and a bovine pericardium collagen membrane (BPCM) group. The control groups comprised a group containing cell culture medium as a negative control group and another positive control group that contained cell cultures. Results: The results revealed no significant difference in MC3T3-E1 cell viability between the treatment and control groups (p < 0.05). Moreover, as observed in the DDMM group, there was an increase in the number of osteoblast cells. Conclusion: DDMM is a suitable alternative biomaterial for GBR as it is non-cytotoxic and could potentially increase the rate of repair of craniofacial defects.
引用
收藏
页码:443 / 449
页数:7
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