Prognostic Correlation Between MFG-E8 Expression Level and Colorectal Cancer

被引：20
作者：
Jia, Min [1 ]
Yao, Huaning [2 ]
Chen, Chao [1 ]
Wang, Yueqin [1 ]
Wang, Han [1 ]
Cui, Tianpen [1 ]
Zhu, Jianhua [1 ]
机构：
[1] HUST, Tongji Med Coll, Lab Clin Immunol, Wuhan Hosp 1, 215 Zhongshan Dadao, Wuhan 430022, Hubei, Peoples R China
[2] HUST, Tongji Med Coll, Dept Pathol, Wuhan Hosp 1, Wuhan, Hubei, Peoples R China
来源：
ARCHIVES OF MEDICAL RESEARCH | 2017年 / 48卷 / 03期
基金：
中国国家自然科学基金;
关键词：
CRC; MFG-E8; Tissue microarray; Immunohistochemistry; Prognosis; GROWTH-FACTOR; 8; APOPTOTIC CELLS; MALIGNANT-MELANOMA; GENE-EXPRESSION; FACTOR-VIII; ANGIOGENESIS; CARCINOGENESIS; PROGRESSION; CARCINOMA; LESIONS;
D O I：
10.1016/j.arcmed.2017.06.003
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background and Aims. Colorectal cancer (CRC) is one of the leading causes of cancer related death all over the world. Milk fat globule-epidermal growth factor (EGF)-factor VIII (MFG-E8) was found to be highly expressed in a variety of cancers. However its role in CRC is unclear. This study investigates the expression of MFG-E8 in CRC tissues and the correlation with clinicopathological features and prognosis in CRC patients. Methods. The expression of MFG-E8 proteins was detected by immunohistochemical staining in 90 samples of CRC. The localization of MFG-E8 in colorectal tumor was examined by immunofluorescence staining The correlation between MFG-E8 protein expression and the clinical pathological features of CRC were evaluated by chi(2) test and Fisher's exact test. The survival rates were analyzed by the Kaplan-Meier method, and the relationship between prognostic factors and patient survival was analyzed by the Cox proportional hazard models. Results. Our results showed that MFG-E8 expression increased significantly in colorectal cancer compared with normal mucosa tissues (p <0.001). We further validated MFG-E8 overexpression in 6 pairs of fresh tumor and adjacent normal mucosa tissues from colorectal cancer patients by Western blot (p <0.05). Immunofluorescence staining showed that MFG-E8 accumulated in close proximity to endothelial cells in human colorectal tumor tissue. In addition, high MFG-E8 protein expression was correlated with lymph node metastasis and some pathological classifications (p <0.05). Furthermore, patients with high protein level of MFG-E8 showed shortened overall survivals (p <0.05). Conclusion. Our results showed that MFG-E8 could be a potential novel prognostic marker for CRC and overexpression of MFG-E8 might be involved in lymph node metastasis and angiogenesis of CRC. (C) 2017 IMSS. Published by Elsevier Inc.
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收藏
页码：270 / 275
页数：6
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