Saccharospirillum mangrovi sp nov., a bacterium isolated from mangrove sediment

A Gram-negative, spirilla, non-spore-forming, motile and strictly aerobic bacterium, designated HK-33(T), was isolated from a mangrove sediment sample in Haikou city, Hainan Province, China. Strain HK-33(T) was able to grow at 10-45 degrees C (optimum 37 degrees C), 0.5-12.0% (w/v) NaCl (2.0 %, w/v) and pH 5.5-8.5 (pH 7.0). The major cellular fatty acids were C-16:0 and summed feature 8 (C-18 : (1)omega 7c and/or C-18 : (1)omega 6c). Ubiquinone-8 was the predominant respiratory quinone, and Q-9 was present in trace amounts. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, two unidentified glycolipids, an unidentified aminophosphoglycolipid and two unidentified lipids. The DNA G+C content was 57.3 mol%. According to 16S rRNA gene sequences similarity, strain HK-33(T) shared 98.6 %, 96.4%, 95.7 and 94.9% sequence similarities to the species Saccharospirillum correiae CPA1(T), Saccharospirillum impatiens EL-105(T), Saccharospirillum salsuginis YIM-Y25(T) and Saccharospirillum aestuarii IMCC 4453(T), respectively. Phylogenetic analysis showed that strain HK-33(T) was clustered with S. correiae CPA1(T), S. impatiens EL-105(T), S. salsuginis YIM-Y25(T) and S. aestuarii IMCC 4453(T). Results of DNA-DNA hybridization analysis revealed that strain HK-33(T) shared 36.3 +/- 1.7% DNA relatedness with S. correiae CPA1(T). On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain HK-33(T) is considered to represent a novel species in the genus Saccharospirillum, for which the name Saccharospirillum mangrovi sp. nov. is proposed. The type strain is HK-33(T) (=KCTC 62178(T) =MCCC 1K03440(T)).