RNA-seq reveals distinctive RNA profiles of small extracellular vesicles from different human liver cancer cell lines

被引：32

作者：

Berardocco, Martina ^{[1
]}

Radeghieri, Annalisa ^{[2
]}

Busatto, Sara ^{[2
]}

Gallorini, Marialucia ^{[3
]}

Raggi, Chiara ^{[4
]}

Gissi, Clarissa ^{[1
]}

D'Agnano, Igea ^{[5
]}

Bergese, Paolo ^{[2
]}

Felsani, Armando ^{[5
,6
]}

Berardi, Anna C. ^{[1
]}

机构：

[1] Spirito Santo Hosp, Lab Stem Cells, UOC Immunohaematol & Transfus Med, Pescara, Italy

[2] Univ Brescia, Dept Mol & Translat Med, Brescia, Italy

[3] Univ G DAnnunzio, Dept Pharm, Chieti, Italy

[4] Humanitas Clin & Res Ctr, Ctr Autoimmune Liver Dis, Rozzano, Italy

[5] CNR, Inst Cell Biol & Neurobiol, Monterotondo, Italy

[6] Genomnia Srl, Bresso, Italy

来源：

ONCOTARGET | 2017年 / 8卷 / 47期

关键词：

extracellular vesicles; liver cancer; microRNA; small nucleolar RNA; RNA sequencing; CIRCULATING TUMOR-CELLS; SMALL NUCLEOLAR RNAS; HEPATOCELLULAR-CARCINOMA; MICRORNA EXPRESSION; GENE-EXPRESSION; EXOSOMES; BIOMARKERS; STABILIZATION; POPULATION; PHENOTYPE;

D O I：

10.18632/oncotarget.20503

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Liver cancer (LC) is one of the most common cancers and represents the third highest cause of cancer-related deaths worldwide. Extracellular vesicle (EVs) cargoes, which are selectively enriched in RNA, offer great promise for the diagnosis, prognosis and treatment of LC. Our study analyzed the RNA cargoes of EVs derived from 4 liver-cancer cell lines: HuH7, Hep3B, HepG2 (hepato-cellular carcinoma) and HuH6 (hepatoblastoma), generating two different sets of sequencing libraries for each. One library was size-selected for small RNAs and the other targeted the whole transcriptome. Here are reported genome wide data of the expression level of coding and non-coding transcripts, microRNAs, isomiRs and snoRNAs providing the first comprehensive overview of the extracellular-vesicle RNA cargo released from LC cell lines. The EV-RNA expression profiles of the four liver cancer cell lines share a similar background, but cell-specific features clearly emerge showing the marked heterogeneity of the EV-cargo among the individual cell lines, evident both for the coding and non-coding RNA species.

引用

页码：82920 / 82939

页数：20

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